溴甲酚紫法测定血清白蛋白临床实用性评价

目的 对溴甲酚紫(BCP)法测定血清白蛋白(ALB)进行方法学评价，探讨其测定结果与传统溴甲酚绿(BCG)法的可转换性。方法 测定BCP法的线性范围、批内与批间变异系数、回收率及临床常见干扰因素对其干扰程度，比较测定结果与BCG法的差异。结果 BCP法测定ALB线性范围可达10～80g／L，批内与批间变异系数分别为0．95％和3．03％，平均回收率为99．4％，252μmol／L的胆红素、4％的Trilitid、4g/L的血红蛋白对其无明显干扰。应用BCP法比传统BCG法平均约低6．0g／L，但两者高度相关(y=1．08x 5．98)。结论 BCP法是一种较BCG法更为特异的测定ALB的方法，利用回归方程可方便地将其转换成BCG法的测定结果，使临床应用这两种方法测定ALB的结果具有可比性。

Evaluation of the clinical applicability of bromcresol purple method for measuring serum albumin.

OBJECTIVE: To evaluate the clinical applicability of bromcresol purple (BCP) method in serum albumin measurement, and to investigate the possibility to render the results thus obtained in agreement with those by traditional bromcresol green (BCG) method. METHODS: The assessment of BCP method was conducted in comparison with BCG method by measuring their linearity ranges, coefficients of variation (CV) in batch and between batches, recovery rates, and resistance to interference by certain normal clinical factors. RESULTS: The linearity of BCP method was 10 to 80 g/L, CV was 0.95% in batch and 3.03% between batches, with an average recovery rate of 99.4%, showing no obvious interference by 252 micromol/L bilirubin, 4% Trilitid, or by 4 g/L hemoglobin separately. Close correlation was observed between the measurement results by the 2 methods (y=1.08x+5.98). The adult reference range was 34-49 g/L by BCP method, which was 6 g/L lower, on average, than that by traditional BCG method. CONCLUSION: BCP method is more specific than BCG method in serum albumin measurement, and the results of the former can be easily and accurately converted so as to be comparable with the results by the latter method.