重症肌无力血清中碳酸酐酶Ⅲ其抗体的检测

目的：检测重症肌无力（MG）患者血清中碳酸酐酶Ⅲ（CAⅢ）及其抗体水平，探讨MG骨骼肌减少的CAⅢ是否参与MG的免疫发病。方法：用Western blot检测18例MG患者、16例其他神经肌肉疾病（ONMD）患者和15名健康人血清中CAⅢ水平；用Western blot与酶联免疫吸附测定（ELISA）检测上述血清中抗CAⅢ的抗体水平。结果：Western blot显示，正常人、MG和ONMD患者血清中都存在CAⅢ，但该蛋白的谱带密度在3组人的血清中差异无统计学意义（P〉0．05）；Western blot与ELISA表明，上述血清中不存在抗CAⅢ的抗体。结论：正常人、MG和ONMD患者血清中均存在CAⅢ蛋白，但不存在抗该蛋白的抗体，说明MG骨骼肌减少的CAⅢ蛋白可能没有直接参与MG的免疫发病。

Detection of Carbonic Anhydrase Ⅲ and Its Antibody in Sera of Myasthenia Gravis

Aim： To detect carbonic anhydrase Ⅲ （CA Ⅲ） and its antibody in sera of myasthenia gravis （MG）, and to explore whether or not the CA Ⅲ protein which was deficient in skeletal muscles of myasthenia gravis （MG） was involved in the immuno-pathogenesis of MG. Methods ： The levels of CA Ⅲ protein in sera from 18 cases of MG, 16 cases of other neuromuscular disorders （ONMD） and 15 cases of healthy volunteers were detected by Western blot, and the antibody against CA Ⅲ in the sera was measured by methods of Western blot and enzyme-linked immunosorbent assay （ELISA）. Results： Immuno-Western blot showed that CA Ⅲ was found in all sera, however, the protein levels were not significant difference between MG and two other groups （P 〉 0.05）. In addition, both Western blot and ELISA demonstrated that there was not the antibody against CA Ⅲ in all sera. Conelusion： There was CA Ⅲ protein, but was not its antibody in all sera. It was suggested that the CA Ⅲ protein which was deficient in skeletal muscle of MG was not directly involved in the immuno-pathogenesis of MG.