仙灵骨葆对大鼠骨质疏松性骨折愈合的影响

背景：骨质疏松可延迟骨折愈合,仙灵骨葆对骨质疏松性骨折愈合的影响及其机制尚有待研究。目的：观察骨质疏松对大鼠股骨干骨折愈合的影响以及仙灵骨葆的干预效果及其作用机制。方法：50只3月龄雌性SD大鼠随机分成5组：正常对照组、骨质疏松组、正常骨折组、骨质疏松性骨折组、治疗组,前2组每组6只,后3组每组12只。去除大鼠双侧卵巢制备骨质疏松模型,8周后制备右侧股骨干中段横行骨折模型。治疗组给予仙灵骨葆灌胃250mg/（kg·d）,正常骨折组、骨质疏松性骨折组灌胃等量生理盐水,灌胃6周。结果与结论：去卵巢后8周骨质疏松组的骨密度显著低于正常对照组（P〈0.05）,说明骨质疏松模型制作成功;灌胃6周,骨质疏松性骨折组骨密度、X射线摄片评分、最大载荷均显著低于正常骨折组和治疗组（P〈0.05）,但给予仙灵骨葆的治疗组仍显著低于正常骨折组（P〈0.05）。以上结果提示骨质疏松大鼠骨折愈合过程延迟,仙灵骨葆对骨质疏松大鼠骨折愈合具有积极促进作用。

Effect of Xianlinggubao on osteoporotic fracture healing in rats

BACKGROUND：Osteoporosis can delay fracture healing.The studies on the effect and underlying mechanisms of Xianlinggubao on osteoporotic fracture healing are unclear.OBJECTIVE：To investigate the effects of osteoporosis on fracture healing of shaft of femur in rats and the intervention effectiveness of Xianlinggubao as well as its underlying mechanisms.METHODS：Fifty female 3 months old Sprague-Dawley rats were randomly divided into five groups：normal control（n=6）,osteoporosis group（n=6）,normal fracture group（n=12）,osteoporotic fracture group（n=12） and Xianlinggubao treatment group（n=12）.Rat model of bilateral ovariectomy was established,and rat model of right shaft femur fracture was prepared after 8 weeks.Rats in the treatment group was given 250 mg/kg Xianlinggubao with intragastric administration,while rats in the normal fracture and osteoporotic fracture groups were given equal volume of normal saline for 6 weeks with intragastric administration.RESULTS AND CONCLUSION：Rats in the osteoporosis group showed markedly decreased bone mineral density,compared to the normal control group at 8 weeks after ovariectomy（P0.05）,which suggested rat models of osteoporosis were prepared successfully.After intragastric administration for 6 weeks,bone mineral density,X-ray film scores and maximal load in the osteoporotic fracture group were significantly lower than those of normal fracture group and Xianlinggubao treatment group（P0.05）.But those in Xianlinggubao treatment group were still markedly lower than those in the normal fracture group（P0.05）.These results suggest that rats with osteoporosis have delayed fracture healing,and Xianlinggubao can promote the fracture healing.