丹参酮的抗抑郁作用研究

目的：研究丹参酮的抗抑郁作用。方法：丹参酮灌胃给药。通过尾静脉注射利血平（2mg·kg-1）复制小鼠抑郁模型,观察眼帘下垂动物数、运动不能动物数,测肛温;腹腔注射丁苯那嗪（40mg·kg-1）复制小鼠抑郁模型,观察眼帘下垂动物数和僵住动物数;尾静脉注射利血平（2mg·kg-1）后进行悬尾、强迫游泳实验,复制小鼠获得性绝望模型,观察小鼠悬尾不动时间和游泳不动时间;通过静脉注射单胺氧化酶抑制剂（盐酸色胺）复制大鼠惊厥模型,观察大鼠动作平均分值、拍打动作持续时间。结果：60、30、20mg·kg-1丹参酮可显著减少利血平复制的抑郁模型小鼠眼帘下垂、运动不能动物数,显著升高模型小鼠肛温（P〈0.05）;60、30mg·kg-1丹参酮可显著减少丁苯那嗪复制的抑郁模型小鼠眼帘下垂、僵住动物数（P〈0.01或P〈0.05）;60、30mg·kg-1丹参酮可显著缩短绝望模型小鼠悬尾不动、游泳不动时间（P〈0.01）;42、21、14mg·kg-1丹参酮可显著降低盐酸色胺复制的惊厥模型大鼠动作平均分,42、21mg·kg-1丹参酮可显著缩短模型大鼠拍打动作持续时间（P〈0.01或P〈0.05）。结论：丹参酮有一定抗抑郁作用。

Study on Anti-depression Effect of Tanshinone

OBJECTIVE： To study anti-depression effect of tanshinone. METHODS：Intragastric administration of Tanshinone. Depression model was induced by injection of reserpine （2 mg·kg-1） via tail vein, and the number of heavy-lidded eyes and akinesia were observed and rectal temperature was determined. Depression model was induced by intraperitoneal injection of tetrabenazine （40 mg·kg-1）, and the number of catalepsy was observed. Mice was injected with reserpine （2 mg·kg-1） via tail vein to conduct tail suspension test and forced swimming test. The time of tail suspension immobility and swimming immobility were observed. The convusion of rats was induced by intravenous injection of MAO inhibitor （tryamine hydrochloride） to observe average value of rat’s action and duration of slapping and beat. RESULTS： Tanshinone 60,30,20 mg·kg-1 could improve heavy-lidded eyes and swimming immobility and rised rectal temperature in depression symptoms of mice induced by reserpine（P0.05）. Tanshinone 60,30 mg·kg-1 could improve heavy-lidded eyes and swimming immobility in depression symptoms of mice induced by tetrabenazine（P0.01,P0.05）. It also shortened the time of tail suspension immobility and swimming immobility in despair symptoms of mice（P0.01）. Tanshinone 42,21,14 mg·kg-1 could depressed actin point in convusions symptoms of rats induced by trypamine hydrochloride. Tanshinone 42,21 mg·kg-1 could shortened pat duriation in convusions symptoms model rats.CONCLUSION：Tanshinone have certain anti-depressant effect.