不同pH值纳米羟基磷灰石复合根充糊剂的体外抑菌性

背景：目前临床应用的根管充填糊剂抗菌作用有限，仃仵组织刺激性强、成形困难、根管封闭性较差及易降解等缺点。目的：比较不同pH值纳米羟基磷灰石复合根充糊刺对感染根臀常见菌的体外抑菌作用。方法：建立体外根管粪肠球菌、白色念球荫感染模型，评价pH8纳米羟基磷灰石糊剂、pH9纳米羟幕磷扶“糊剂、pH10纳米黔基磷灰钉糊刑存离体牙根管内的抗菌作用，并以氧化锌丁香油做对照。结果与结论：所有根充糊剂均其有抑荫作用。各pH值纳米砼基磷灰石糊剂对粪肠球菌抑制作用实验组优于氧化锌丁香油组（P〈0．05），不同pH值纳米羟慕磷灰石纰组间比较差异无显著性意义（P〉0．05）。而对白色念珠芮抑制作用各pH值纳米羟基磷灰石组不及氧化锌丁香油组（P〈0．05），各pH值纳米羟基磷灰石组组间比较，pH10纳米羟基磷灰石组〉pH9纳米羟堆磷灰秆组〉pH8纳米羟基磷灰石组（P〈0．05）。结果表明，感染根管模型中，纳米羟基磷灰石根充糊剂对粪肠球菌的抑制作用好于氧化锌丁香油（P〈0．05），而对白色念珠菌的抑制作用不及氧化锌丁香油（P〈0．05）。pH=10纳米胫基磷灰石复合根充糊剂的抑菌作用最佳。

Antimicrobial activity of nano-hydroxyapatite composite root canal filling materials with different pH values in vitro

BACKGROUND： The antimicrobial activity of root canal filling materials in clinical application is limited at present. Root canal filling materials have detects, such as strong stimulation to tissues, difficulty in formation, bad root canal sealing and easy to be degradable. OBJECTIVE： To study the effect of antimicrobial activity of nano-hydroxyapatite composite root canal filling materials with different pH values on common bacteria infected root canal in vitro. METHODS： In vitro infected root canal model of enterococcus faecalis and candida albicans was established. The antibacterial property of nano-hydroxyapatite paste with different pH values in root canal in vitro was evaluated, including pH 8, pH 9 and pH 10. Zinc oxide eugenol were as a control. RESULTS AND CONCLUSION： All the root canal filling materials have bacteriostatic effect. Compared with the control group, the inhibiting effect of nano-hydroxyapatite paste in each pH value group on enterococcus faecalis was better than that in the control group （P 〈 0.05）. There was no significant difference among nano-hydroxyapatite paste in each pH value group （P 〉 0.05）. The inhibitory effect of nano-hydroxyapatite paste in each pH value group on candida albicans was not better than that in the control group （P 〈 0.05）. Nano-hydroxya paste in each pH value group was compared, and pH 10 group 〉 pH 9 group 〉 pH 8 group （P 〈 0.05）. These findings suggest that in the model of infected root canal, the inhibiting effect of nano hydroxyapatite paste on enterococcus faecalis was better than that of zinc oxide eugenol （P 〈 0.05）, but the inhibiting effect of nano-hydroxyapatite paste on candida albicans was not better than that of zinc oxide eugenol （P 〈 0.05）. Besides, nano-hydroxyapatite composite root canal filling materials with pH=10 have the best bacteriostatic property.