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| 有机磷诱导HUVEC-12损伤的实验研究 | |
| 引用本文： | 李荣霏,易高众,郭鹏程,谢利,黄红林. 有机磷诱导HUVEC-12损伤的实验研究[J]. 中国实用医药, 2008, 3(23): 30-31 |
| 作者姓名： | 李荣霏 易高众 郭鹏程 谢利 黄红林 |
| 作者单位： | 1. 湖南省怀化医专药理学教研室,418000 2. 怀化第一人民医院呼吸内科 3. 衡阳,南华大学药物药理研究所,湖南 |
| 摘 要： | 目的观察有机磷农药敌百虫对人脐静脉内皮细胞（HUVEC-12）损伤的时相特征,探讨敌百虫诱导内皮细胞损伤的作用机制。方法将HUVEC-12与含不同浓度敌百虫的DMEM培养基孵24h后,MTT法检测细胞的活性;以IC50浓度的敌百虫处理细胞一定时间后（0、4、6、8、12、24、48h）,观察敌百虫诱导内皮细胞损伤的时相特征;硫代巴比妥法检测细胞上清液中丙二醛（MDA）浓度。结果敌百虫呈时间依赖性地促进内皮细胞凋亡及细胞上清液MDA产生。结论敌百虫刺激HUVEC-12氧化应激,致细胞损伤。 |
| 关 键 词： | 敌百虫 人脐静脉内皮细胞 丙二醛 内皮型一氧化氮合酶 |
The damage on human umbilical vein endothelial cell lines induced by trichlorfon |
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| LI Rong-fei,YI Gao-zhong,GUO Peng-cheng,et al.. The damage on human umbilical vein endothelial cell lines induced by trichlorfon[J]. China Practical Medical, 2008, 3(23): 30-31 | |
| Authors: | LI Rong-fei YI Gao-zhong GUO Peng-cheng et al. |
| Affiliation: | LI Rong-fei,YI Gao-zhong,GUO Peng-cheng,et al.Department of Pharmacology,Huaihua Medical College,Huaihua 418000,China |
| Abstract: | Objective To explore the time-effect of damage in human umbilical vein endothelial cell lines induced by Trichlorfon and to analyze its mechanism. Methods HUVECs-12 were cultured in DMEM containing Trichlorfon for 24 h, the growth inhibition was measured by MTT assay. Cells were incubated in IC50 concentration Trichlorfon （400 μmol/L） for different times（0,4,6,8,12,24,48 h） ） to test the time-effect of damage in human umbilical vein endothelial cells ; the level of malondialde-hyde （MDA） in supernatant-condi-tioned media was monitored by thiobituric acid reaction （TBA）. Results Trichlorfon inhibited HUVECs-12 growth in a dose-dependent manner and increased the production of MDA. Conclusion Trichlorfon can induce damage in human umbilical vein endothelial cells. |
| Keywords: | Trichlorfon Human umbilical vein endothelial cells Malondialdehyde Endothelial nitric oxide synthase |
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