腹泻患者粪便标本中A组轮状病毒感染特征分析

目的:分析病毒性腹泻患者中人轮状病毒(Human Rotavirus,HRV)的感染情况及毒株流行特点。方法:收集杭州市239份疑似病毒性腹泻患者粪便标本分别应用酶联免疫吸附法及RT-PCR法进行HRV抗原及核酸检测。。其中HRV阳性核酸应用巢式RT-PCR进行轮状病毒分型检测。结果:239份粪便标本中HRV抗原阳性为44份(18.4%),HRV核酸阳性为55份(23.0%),其中G血清分型:G3型15份(27.3%),G1型9份(16.4%),G2型为7份(12.7%),G9型为1份(1.8%),G2+G3混合感染1份(1.8%),未分型22份(40%)。结论:A组轮状病毒是婴幼儿病毒性腹泻的重要病原体,同时也可引起成人腹泻散发流行,成人A组轮状病毒流行季节和流行株与婴幼儿一致,G3为主要流行株,且同时存在多种血清型流行,未分型标本的比例较大有待深入研究。

Study on epidemiological characteristics of cluster A rotavirus from stool specimens of patients with acute diarrhea

Objective:To analyze the infectious status and epidemiological characteristics of acute viral diarrhea caused by cluster A human rotavirus.Methods:A total of 239 stoo1 specimens from suspected cases with acute virus diarrhea were collected in Hangzhou.IDEIATM kits of Lanzhou institute for biological products were used for antigen detection of human rotavirus.The nucleic acid identifications of the rotavirus were determined by RT-PCR,and the positive samples of HRV nucleic acid were analyzed for genotypes by nested-PCR.Results:Among 239 detected stoo1 specimens,rotavirus antigen positive was 44 cases(18.4%).Rotavirus nucleic acid positive was 55 cases(23.0%),the serotypes were G3(15 cases,27.3%),G1(9 cases,16.4%),G2(7 cases,12.7%),G9(1 case,1.8%),G2+G3(1 case,1.8%) and unidentified ones(22 cases,40%).Conclusion:Cluster A rotavirus remained the most important pathogen causing diarrhea among infants and sporadic epidemic case with acute diarrhea among adults.The epidemic season and serotype of group A rotavirus in adults are all just the same as those in infant and children.G3 was the most prevalent serotype,at the same time,there are many serotypes popular.The non-typeable specimens accounted for larger proportion,need further research.