| 蒽酮-硫酸法测定维地红涂剂中多糖的含量 |
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| 引用本文： | 汪依林,常明泉,陈 芳,等. 蒽酮-硫酸法测定维地红涂剂中多糖的含量[J]. 海峡药学, 2014, 0(3): 54-55 |
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| 作者姓名： | 汪依林 常明泉 陈 芳 等 |
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| 作者单位： | 湖北医药学院附属太和医院,十堰442000 |
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| 基金项目： | 湖北省重大科学技术鉴定成果（登记号：EK2012D150018000359）：太和科研基金资助项目（2009D34） |
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| 摘 要： | 目的：应用蒽酮-硫酸法测定维地红涂剂中多糖的含量。方法以无水葡糖糖为对照品，0.2％蒽酮-硫酸试液为显色剂，检测波长620nm。结果多糖在1.9～34.2μg· mL -1范围内线性关系良好，y＝0.033X-0.0067（r＝0.9999），回收率96.0％，RSD＝1.96％（n＝6）。结论该方法用于维地红涂剂中多糖的含量测定方法稳定，结果重现性好。
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| 关 键 词： | 维地红涂剂 多糖 蒽酮-硫酸试液 含量测定 |
Determination of polysaccharide in Verdihong Paint by anthrone-sulfuric acid method |
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| WANG Yi-lin,CHANG Ming-Quan,CHEN-Fang,WANG Gang,CHEN Li. Determination of polysaccharide in Verdihong Paint by anthrone-sulfuric acid method[J]. Strait Pharmaceutical Journal, 2014, 0(3): 54-55 |
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| Authors: | WANG Yi-lin CHANG Ming-Quan CHEN-Fang WANG Gang CHEN Li |
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| Affiliation: | (Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan ,442000, China) |
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| Abstract: | OBJECTIVE To detemine the content of polysaccharide in Verdihong paint by the anthrone sulfuric acid method.METHODS Using the anhydrous dextrose as the control ,0.2%Anthrone-Sulfuric acid as the stain , The content of Polysaccharide was determined by ultraviolet spectrophotometry at the wavelength of 620 nm.RESULTS The polysaccharide curve was linear within a range of 1.9-34.2μg · mL-1 , linear equation was y =0.033 x -0.0067 （ r =0.9999 ） , the average recovery was 96.0% with RSD of 1.96%（ n =6 ）.CONCLUSION This method is accurate and the results has fine repetition. |
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| Keywords: | Verdihong paint Polysaccharide Anthrone-sulfuric acid method Content determination |
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