脐带间充质干细胞移植对胶原诱导型关节炎大鼠炎性趋化因子表达的影响

目的观察脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSC)移植对胶原诱导型关节炎(collagen typeⅡ-induced arthritis,CIA)大鼠模型血清中:血管细胞间黏附分子-1(VCAM-1)、基质细胞衍生因子-1(SDF-1)、内皮细胞生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)表达水平的影响,初步探讨UC-MSC移植对CIA大鼠炎性趋化因子的调节作用。方法采用鸡Ⅱ型胶原加完全弗氏佐剂建立CIA大鼠模型,经大鼠尾静脉注射UC-MSC;以酶联免疫吸附试验(ELISA)检测各组大鼠干预前后血清VCAM-1、SDF-1、VEGF、TNF-α水平变化。结果模型组血清VCAM-1、SDF-1、VEGF、TNF-α水平均明显高于同期空白对照组(P<0.05);经UC-MSC治疗组上述炎症因子水平逐渐降低,至第5周上述指标均接近空白对照组(P<0.05)。结论 CIA大鼠存在免疫功能紊乱,其炎症趋化因子水平显著增高;UC-MSC能下调CIA大鼠炎症趋化因子水平,减轻机体免疫性炎症反应,促进疾病缓解,改善预后。

The Immunomodulation Effect of UC-MSC on the Inflammatory Chemokines of Rats with Collagen Type II Induced Arthritis

Objective To observe the immunomodulation effects of umbilical cord mesenchymal stem cells （UC-MSC） on the VCAM- 1, SDF- 1, VEGF, TNF- α of rats with collagen Ⅱ induced arthritis （CIA）. Methods First,The rats were immunized by intradermal injection of chickencollagen type 11 emulisified with complete Freund＇ s adjuvant （CFA）. The swelling of foot , hair color , and action state of the rats were observed after injecting UC-MSC by caudal vein,the rats were scored with the arthritis index（AI） once a week. Then, The CD 4, CD 25 of peripheral blood lymphocytes and the CD 11 b of peripheral blood neutrophil were detected by flow cytometry（FCM） ;The concentration of IL-17 in serum was detected by ELISA. Results Obvious swelling of the feet were found in the experiment group compared with norma group. ELISA the con- centration of VCAM- 1, SDF- 1, VEGF, TNF-α in serum of the experiment group are higher than normal group （ P 〈 0.05 ）. After UC- MSC treatment, these factors level were significantly difference than the model group （ P 〈 0.05 ）, and till to the fifth week these factors in the UC- MSC group were mostly the same as normal group. Conclusion UC-MSC can inhibit the development of CIA rats＇disease and reduce the immunity inflammatory reaction, to induce immunological reconstitution. UC-MSC also can lighten the CIA rats joint damage by repairing the vascular endothelial cells and synovial cells.