Fhit在鼻腔鼻窦恶性肿瘤中表达的研究

目的：研究Fhit基因在鼻腔鼻窦恶性肿瘤中的表达及临床意义，探讨其相关性。方法：采用免疫组织化学SP法检测48例原发性鼻腔鼻窦恶性肿瘤组织、29例癌旁正常组织及21例良性病变组织中Fhit基因的表达；采用碘化丙啶（PI）及苏木精一伊红染色检测肿瘤细胞自发性凋亡情况。结果：48例中，26例（54．17％）Fhit表达减少或缺失，22例（45．83％）表达正常。Fhit表达的平均A值与凋亡指数（AI）成正相关（r=0.379，P〈0.01）。Fhit表达正常者预后显著好于表达减少或缺失者（P〈O.05）。结论：Fhit基因失活与鼻腔鼻窦恶性肿瘤的发生、预后相关，可能是该肿瘤的抑癌基因，其抑癌作用可能通过促进细胞凋亡实现，Fhit基因表达减弱有可能成为鼻腔鼻窦恶性肿瘤新的预后指标。

Fhit expression and its relationship with apoptosis and prognosis in nasal and paranasal sinus carcinomas

Objective:To assess the protein expression of Fhit and its correlation with spontaneous apoptosis, tumor clinicopathological parameters and prognosis in nasal and paranasal sinus carcinomas.Method:Forty-eight patients with NPSC treated by radical operations from 1991 to 2000 were studied retrospectively. Patients included 23 female and 25 male ranging in age from 20 to 71 years. Spontaneous apoptotic cells were visualized by TUNEL accompanied by HE and PI stain. Immunohistochemistry using SP method was used to assess the expression of Fhit. Result:The loss of expression of Fhit was found in 26 patients(54.17%,26/48).Comparing with abutting non-neoplastic tissue, reduced expression of Fhit was found in 16(16/29, 55.17%) patients. The adenoid cystic carcinoma showed stronger expression of Fhit than squamous cell carcinomas(P<0.05).The expression of Fhit correlated positively with apoptosis index(r=0.379,P<0.01).Patients with positive expression of Fhit had much better prognosis than those with abnormal expression (P<0.05).Conclusion:The reduced expression of Fhit may be associated with carcinogenesis and prognosis of nasal and paranasal sinus carcinomas. It could be the tumor suppressor gene and realize its function through accelerating apoptosis in this kind of neoplasm. Fhit expression may provide a novel predictive indicator for disease-free survival after surgical resection and postoperative radiochemotherapy in nasal and paranasal sinus carcinomas.