先兆子癎患者胎盘组织滋养层细胞差异表达蛋白初步研究

目的：分析先兆子癎患者胎盘组织滋养层细胞差异表达蛋白,探讨差异蛋白与疾病发生的可能关系,为研究先兆子癎发病机制奠定基础.方法：分离、纯化、鉴定5例正常妊娠妇女和5例先兆子癎患者胎盘组织滋养层细胞,液氮反复冻融研磨提取细胞总蛋白.双向电泳技术对总蛋白进行分离,银染显色,获得蛋白质图谱.用PDQuest软件进行图像分析,寻找差异蛋白.结果：正常妊娠及先兆子癎患者胎盘组织滋养层细胞总蛋白双向电泳各重复3次,得到重复性好的蛋白图谱.PDQuest软件进行图像分析发现,先兆子癎患者胎盘滋养层细胞有34种蛋白在质或量上存在表达增高或减少.结论：先兆子癎患者胎盘组织滋养层细胞中存在差异蛋白,这些蛋白在先兆子癎的发生过程中可能有重要作用.

Proteomic analysis in placental trophoblasts of pre-eclampia patients

Objective: To identify differential expression of proteins in placental trophoblasts in order to clarify the pathology and etiology of pre-eclampsia(PE).Methods: The trophoblasts from human normal and PE placentas were isolated,purified and identified.Proteins in normal and PE samples underwent two-dimensional polyacrylamide gel electrophoresis(2-D PAGE) and silver stained gel imaging with PDQuest image analysis software.Results: Comparative proteomic analysis showed 34 differential protein spots.Conclusion: Our results suggest that these proteins are differentially expressed in PE,and may play an important role in the pathogenesis of PE.Furthermore,these proteins could be useful biomarkers for PE.