| 以hTERT基因反义核酸抑制端粒酶活性增加白血病细胞对CDDP诱导凋亡的敏感性 |
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| 引用本文： | 张洹,何冬梅.以hTERT基因反义核酸抑制端粒酶活性增加白血病细胞对CDDP诱导凋亡的敏感性[J].中德临床肿瘤学杂志,2004,3(1):42-46. |
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| 作者姓名： | 张洹 何冬梅 |
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| 作者单位： | 广州暨南大学医学院血液病研究所 510632
(张洹),广州暨南大学医学院血液病研究所 510632(何冬梅) |
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| 基金项目： | This project was supported by grants from Foundation of Science and Technology of Guangzhou city (2001-Z-037-01) and Guangdong Province (021195). |
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| 摘 要： | 目的 以hTERT反义核酸抑制白血病细胞(HL-60和K562)端粒酶活性,研究CDDP诱导凋亡敏感性的变化。方法 全硫代反义核酸由上海生物化学研究所合成和纯化;端粒酶活性用试剂合测定(宝灵曼公司产品);用形态学方法和流式细胞仪检测细胞调亡。结果实验结果显示,hTERT全硫代反义核酸,通过下调hTERT基因表达,显著地抑制端粒酶活性;端粒酶活性下降以后,白血病细胞对CDDP诱导调亡的敏感性显著升高。结论 以hTERT基因反义核酸抑制端粒酶活性增加白血病细胞对CDDP诱导凋亡的敏感性。
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| 关 键 词： | hTERT基因 反义核酸 端粒酶 白血病 CDDP 诱导凋亡 敏感性 化疗 流式细胞仪 |
| 收稿时间： | 11 February 2003 |
Inhibition of Telomerase with hTERT Antisense Increases
Susceptibility of Leukemic Cells to CDDP-induced Apoptosis |
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| Zhang?Yuan?Email author,HE?Dongmei.Inhibition of Telomerase with hTERT Antisense Increases
Susceptibility of Leukemic Cells to CDDP-induced Apoptosis[J].The Chinese-German Journal of Clinical Oncology,2004,3(1):42-46. |
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| Authors: | Email author" target="_blank">Zhang?Yuan?Email author HE?Dongmei |
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| Institution: | (1) Institute of Hematology, Medical College of Jinan University, 510632 Guangzhou, China |
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| Abstract: | To investigated the effectof inhibition of telomerase with hTERT antisense on leukemic cells(HL-60 and K562) to CDDP-induced apoptosis. Antisense phosphorothioateoligodeoxynucleotide (AS PS-ODN) was synthesized and purified.Telomerase activity was detected by Telomerase PCR ELASA kit andcell apoptosis was observed by morphological method and determinedby flowcytometry. AS PS-ODN couldsignificantly inhibit telomerase activity by down regulating thehTERT expression, and increase the susceptibility of leukemiccells to CDDP-induced apoptosis. Inhibition of telomerase with hTERT antisense can increases the susceptibility of leukemic cells to CDDP-induced apoptosis. |
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