蛋白质组学技术用于功能性胃肠病研究

目的:探讨将蛋白质组学方法用于功能性胃肠病发病机制研究的可行性。方法:选择发病率较高的肠易激综合征(irritable bowel syndrome,IBS)亚型患者作为研究对象,运用蛋白质组学方法,双向凝胶电泳后应用不同染色方法进行染色,Image Master 2D Elite分析软件对图像进行分析。结果:考马斯亮蓝法染色用于腹泻型IBS患者,平均得到蛋白质点426个;硝酸银染色法用于便秘型IBS患者,平均得到蛋白点581个。结论:双向凝胶电泳法可得到IBS患者结肠黏膜组织的蛋白质组图谱;硝酸银染色法的分辨率明显高于考马斯亮蓝法。

Applying the proteomical technology into the research of functional gastrointestinal disor-ders

Objective:To explore the feasibility of research the pathophysiology of functional gastrointestinal disorders with proteomical methods.Methods: Different IBS(irritable bowel syndrome,IBS) subtype patients were chosen to study.Proteomical methods were used to explore.Performing two dimensional gel electrophoresis followed with different staining methods,analyzing the gel maps with software Image Master 2D Elite.Results: Average protein spots were 426 in the diarrhoea-predominant IBS group by using Coomassie brilliant blue staining,while the constipation-dominant IBS group by using silver staining got average 581 protein sptos.Conclusion: By using the two dimensional gel electrophoresis technique,proteome maps of human colonic mucosa were obtained.The resolution of silver staining is higher than that of the Coomassie brilliant blue staining.