燕麦β-葡聚糖对小鼠结肠菌群及其功能的影响

目的:研究燕麦β-葡聚糖对小鼠结肠菌群及其功能的影响。方法:体重23～25g的健康昆明种小鼠126只,分成7组,每组18只,实验组(1～6组)分别以高低剂量0.5g和0.25g/(㎏bw·d)灌胃给予燕麦β-葡聚糖OG2600、OG340和OG5,对照组给予相同体积的生理盐水,灌胃期28d。分别于实验14、28和35d(停止灌胃后1w)分析各组小鼠结肠双歧杆菌、乳酸杆菌和大肠杆菌的变化;并在实验28d用气相色谱法测定小鼠结肠中短链脂肪酸的含量;用流式细胞仪分析结肠粘膜上皮细胞变化。结果:与对照组比,随着灌胃时间的延长,各实验组均可使小鼠肠道和粪便中的双歧杆菌和乳酸杆菌增殖,使大肠杆菌数量减少,作用效果与分子大小和剂量有关;第2、4、6组小鼠结肠内容物的乙酸、丙酸、丁酸的量显著高于对照组(P<0.05);第6组细胞增殖指数和对照组有显著差异(P<0.05)。结论:燕麦β-葡聚糖具有调节小鼠肠道菌群的作用;在体内容易发酵产生较高比例的短链脂肪酸,能促进结肠粘膜上皮细胞的增殖。

EFFECT OF OAT β-GLUCAN ON COLON FLORA AND ITS FUNCTION IN MICE

Objective: To investigate the effect of oatβ-glucan on colon flora and its function in mice. Method:126 Kunming mice(23-25g)were divided into seven groups (18 mice in each). The group 1 and 2, 3 and 4 ,5 and 6 were respectively administered oatβ-glucan (OG2600 , OG340 and OG5) at dose of 0.25 and 0.5g/(kg bw·d)daily for 28 d. The control group received the equal volume of normal saline. Bifidobacterium , Lactobacillus and Enterobacillus were monitored in colon after oral administration of oat β-glucan at 14 d, 28 d and 35 d (after terminating oatβ-glucan admimistration for 1 w) . The contents of short chain fatty acids (SCFA) in colon were analyzed by GC and cell proliferation in colonic mucosa epithelium by FCM in each group. ResuIts:The numbers of Bifidobacterium , Lactobacillus compared with the control were significantly increased and Enterobacillus strains were decreased in experimental groups. Oat β-glucan had the function of regulating intestinal flora and the effect was related to molecular weight and dosage. The contents of acetate, propionate, butyrate were higher in the group 2, 4 and 6 compared with the control (P<0.05)in colon. The proliferation of the colonic mucosa cells in group 6 was significantly higher than that in the control group (P<0.05). Conclusion:Oat β-glucan can regulate colon flora and enhance the proliferation of the colonic mucosa cells.