雌二醇对大鼠肝星状细胞MMP-2、TIMP-1表达的影响 |
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引用本文: | 周定辉,谢建萍,周建亮,黄柳云.雌二醇对大鼠肝星状细胞MMP-2、TIMP-1表达的影响[J].国际医药卫生导报,2009,15(9):9-12. |
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作者姓名: | 周定辉 谢建萍 周建亮 黄柳云 |
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作者单位: | 邵阳市中心医院感染病科,422000 |
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摘 要: | 目的探讨雌二醇对肝星状细胞(HSC)的影响及其可能的作用途径。方法培养大鼠HSC,初次传代后,HSC随机分为4组,分别加入不同浓度的雌二醇,噻唑蓝(MTT)法检测雌二醇对HSC增殖的影响;逆转录-聚合酶链式反应(RT—PCR)分析其对HSC的基质金属蛋白酶因子(MMP-2)、组织金属蛋白酶抑制剂(TIMP-1)的mRNA表达的影响;免疫细胞化学分析其对HSC的α—SMA蛋白的表达的影响。结果MTT法发现雌二醇抑制HSC的增殖,并且随浓度增加抑制作用增强,与阳性对照组比较雌二醇干预组HSCMMP-2mRNA表达增加(P〈0.01),TIMP—1mRNA的表达下降(P〈0.01),α-SMA蛋白表达明显下降(P〈0.05)。结论雌二醇具有抑制肝纤维化作用是因为:①可以抑制HSC的增殖、活化;②促进MMP-2mRNA的表达,抑制了TIMP-1mRNA的表达。
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关 键 词: | 雌二醇 肝星状细胞 基质金属蛋白酶 组织金属蛋白酶抑制剂 肝纤维化 |
The effect of estradiol on the expression of MMP-2 and TIMP-1 in rat hepatic stellate cells |
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Institution: | ZHOU Ding-hui, XlE Jian-ping, ZHOU Jian-liang, et al.(Department of infectious disease, Shaoyang Central hospital, Shaoyang 422000, China) |
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Abstract: | Objective To analyze the effect and the pathway of estradiol on hepatic stellate cells (HSC). Methods Cultured HSC were randomly divided into 4 groups and different concentrations of estradiol were added, then the effect of estradiol on the proliferation and the expression of MMP-1 and TIMP-1 mRNA, and α-SMA protein were detected by MTT assay, RT-PCR and Immunocytochemistry, respectively. Results The results showed that estradiol could inhibit HSCs proliferation in the dose-dependent manner. We found that estradiol up regulated the expression of MMP-2 (P<0.01) and down-regulated the expression of TIMP-1 in mRNA level (P <0.01). The expression of α-SMA protein significantly decreased as compared with the negative control (P<0. 05).Conclusion Estradiol has anti-fibrosis function. This maybe related to increase the expression of MMP-2 and decrease the expression of TIMP-1. |
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Keywords: | Estradiol Hepatic stellate cell MMP-2 TIMP-1 Liver fibrosis |
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