子宫内膜癌细胞凋亡与p53蛋白表达和细胞增殖的相关性研究

目的 探讨子宫内膜癌组织中细胞凋亡与p53蛋白表达和细胞增殖的相关性，分析这些指标与子宫内膜癌组织学类型和恶性程度的关系。方法 采用DNA末端转移酶介导的Bio-dUTP原位末端标记技术、免疫组化技术及HE染色，对细胞凋亡与p53蛋白表达和增殖细胞核抗原（PCNA）进行原位观察和相关性研究。结果 64例子宫内膜癌细胞中细胞凋亡密度均低于对照组，并随肿瘤恶性程度增高及p53蛋白表达增强而降低。64例子宫内膜癌中30例（46.9%）p53蛋白呈阳性表达，阳性细胞含量随肿瘤恶性程度升高而增加，对照组均呈阴性。子宫内膜癌组织中增殖细胞核抗原高于对照组，并随肿瘤恶性程度的增高及p53蛋白表达增强而增加。结论 子宫内膜癌p53蛋白表达和功能异常与其细胞增殖及凋亡失衡有关。

Correlation Between Apoptosis and Abnormal Protein p53 Expression and Proliferation in the Endometrial Carcinoma

Objective To explore the effect of p53 protein expression in endometrial carcinama on the cell prolieration and apoptosis,and its correlation to histological classification and malignant degree.Methods 64 cases of endometrial carcinoma and 10 normal endometrial tissues were enclosed into the study.p53 protein expression,proliferating cell nuclear antigen(PCNA) and cell apoptosis were observed in situ with TUNEL technique,immunohistochemical staining and HE staining.Results Protein p53 expression was found in 46.9% of carcinoma group,the numbers of positive cell were increased with malignant degree increased.None of the controls was positive.The density of apoptosis cell in the carcinoma was lower than in controls,and it was negatively correlated to p53 expression and malignant degree.Compared with controls,the PCNA expression in carcinoma tissues was higher and it was increased with enhanced p53 expression and the malignant degree.Conclusion p53 expression and its abnormal function may be associated with the imbalance between cell proliferation and the cell apoptosis.