难治性癫痫患者致痫灶细胞凋亡现象的研究

目的观察难治性癫痫患者手术切除癫痫灶中细胞凋亡现象,探讨神经元凋亡在癫痫发病机制中的意义,为临床治疗提供新的理论依据.方法收集16例经手术治疗的难治性癫痫患者致痫灶标本,在对临床资料全面分析的基础上,应用光镜、电镜及凋亡细胞DNA原位末端标记法（TUNEL）观察标本中存在的细胞凋亡现象.结果致痫灶周围神经元普遍固缩、减少,反应性胶质细胞增生.TUNEL阳性细胞数量较对照组显著增高（P＜0.01）,且绝大部分为神经元.结论难治性癫痫患者致痫灶周围存在细胞凋亡现象,神经元凋亡可能参与癫痫的发病过程.

Study on apoptosis in epileptic focus of patients with intractable epilepsy

Objective To observe apoptosis in surgically resected epileptic focus of patients with intractable epilepsy and explore its roles in epileptic pathogenesis,in order to provide a fresh theory for clinical therapy.Methods The specimens from 16 patients with intractable epilepsy were collected.With light electron microscope and TUNEL method,apoptosis were observed.Results The loss and pyknosis of neurons and the presence of reactive astrocytes seemed to be a common pathological characteristics.There was a significant difference(P<0.01) in the number of TUNEL positive cells,most of which were neurons,between the patients with intractable epilepsy and the controls.Conclusion Apoptotic neurons appeared around epileptic focus of patients with intractable epilepsy,and apoptosis may relate to the pathogenesis of epilepsy.