| 基因释放剂制备结核杆菌热休克蛋白70(hsp70)DNA模板与传统制备方法比较 |
| |
| 引用本文： | 陈丽,史小玲,张建军,李晖. 基因释放剂制备结核杆菌热休克蛋白70(hsp70)DNA模板与传统制备方法比较[J]. 泸州医学院学报, 2005, 28(2): 130-131 |
| |
| 作者姓名： | 陈丽 史小玲 张建军 李晖 |
| |
| 作者单位： | 1. 泸州医学院公共卫生系
2. 泸州医学院附属医院传染病科,四川泸州,646000 |
| |
| 摘 要： | 目的:比较常规试剂盒和使用基因释放剂提取结核杆菌热休克蛋白70(hsp70)DNA模板的质量,为进一步获得目的基因优化方案.方法:提取结核杆菌DNA阶段,分别使用常规试剂盒和基因释放剂,然后采用相同的PCR方案进行扩增,溴化乙锭染色,紫外光下观察目的基因扩增效果.结果:常规试剂盒提取结核杆菌DNA,未能扩增出相应的目的基因;而使用基因释放剂,则扩增效果良好.结论:常规试剂盒相比,基因释放剂在提取结核杆菌DNA时,能够制备良好的模板,为PCR扩增目的基因奠定基础.
|
| 关 键 词： | 基因释放剂 结核杆菌 |
| 文章编号： | 1000-2669(2005)2-0130-02 |
| 修稿时间： | 2004-11-08 |
AMPLIFICATION OF HSP 70 DNA TEMPLATES OF M.TUBERCULOSIS WITH GENERELEASER |
| |
| Chen Li,et al. AMPLIFICATION OF HSP 70 DNA TEMPLATES OF M.TUBERCULOSIS WITH GENERELEASER[J]. Journal of Luzhou Medical College, 2005, 28(2): 130-131 |
| |
| Authors: | Chen Li et al |
| |
| Affiliation: | Chen Li,et alDepartment of Prophylactic Medicine,Luzhou Medical College |
| |
| Abstract: | Objective: To Compare the qu a liy of the DNA templates of hsp 70 extracted from M. Tuberculosis with tradition al kit or genereleaser. Methods: The extracted DNA from M. Tuberculosis with traditional kit or genereleaser were subjected to the same PCR program, and then the PCR products were observed under ultra violet rays. Results: The target gene fragment was not seen when the te mplate was prepared with traditional kit, while it was clearly observed when pre pared with generaleaser. Conclusions: Genereleaser is he lpful in preparing higher quality temaplate from M. Tuberculosis compared with t radional kit. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
