使用GEO数据集分析驱动蛋白KIF2C在膀胱癌中的表达及临床意义

目的 通过基因表达汇编公共数据库，探讨驱动蛋白家族成员2C（kinesin family member 2C，KIF2C）在膀胱癌患者中的表达，探索其表达与临床病理特征的联系，评价KIF2C对膀胱癌术后患者预后评估的意义。方法 检索并下载美国国立生物技术信息中心的肿瘤公共数据集，对表达谱资料和临床信息进行分析，利用基因集富集分析受KIF2C调控的相关基因集。结果 KIF2C在膀胱癌组织中的表达水平高于其在正常膀胱组织中的表达(P<0.000 1)。膀胱癌患者疾病进展、T分期、N分期和肿瘤分级，KIF2C的高、低表达组之间均有显著性差异(P<0.05)。KIF2C高表达患者的肿瘤特异性生存期及总生存期显著低于低表达患者(P<0.05)。KIF2C高表达样本富集了与核糖体蛋白质、多梳蛋白、内源性核糖核酸酶、E2F转录因子、抑癌基因RB有关的基因集。结论 KIF2C与膀胱癌的多个病理指标相关，有作为判断膀胱癌患者预后的标志物和治疗肿瘤的靶标的潜力。

Analysis of clinical significance of expression of KIF2C in bladder cancer utilizing GEO datasets

Objective To investigate the expression of kinesin family member 2C (KIF2C) in bladder cancer (BC) utilizing GEO datasets, to clarify the relationship between KIF2C expression and clinicopathological characteristics of patients with BC, and to evaluate the possibility of using KIF2C as a prognosis marker in BC. Methods BC gene expression studies were collected and the relationship between expression level of KIF2C and clinical information were analyzed. Gene sets enrichment analysis (GSEA) was conducted to explore the gene sets enriched in KIF2C high-expression samples. Results The expression of KIF2C was up-regulated in BC (P<0.000 1); KIF2C expression was significantly associated with progression, grade, T stage, and N stage. Higher expression of KIF2C indicated poor prognosis in BC. GSEA indicated that KIF2C regulated gene sets associated with ribosomalprotein, polycombprotein, endogenous ribonuclease, E2F, and anti-oncogene RB. Conclusion KIF2C is highly expressed in BC and functions as a potential marker and target for diagnosis and treatment of BC.