帕瑞昔布对缺血再灌注后肺组织血红素加氧酶-1表达水平及凋亡程度的影响* |
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引用本文: | 吕强,刘宇,胡云霞,李美亭.帕瑞昔布对缺血再灌注后肺组织血红素加氧酶-1表达水平及凋亡程度的影响*[J].中国现代医学杂志,2019,29(11):14-18. |
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作者姓名: | 吕强 刘宇 胡云霞 李美亭 |
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作者单位: | (四川省医学科学院·四川省人民医院 麻醉科,四川 成都 610072) |
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基金项目: | 四川省卫生厅科研项目(No:110233) |
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摘 要: | 目的 探讨不同剂量帕瑞昔布对大鼠肺缺血再灌注(IR)后肺组织内血红素加氧酶-1(HO-1)表达水平及凋亡程度的影响。方法 随机将40只雄性SD大鼠(280~300?g)分为5组:对照组(C组)、缺血再灌注组(IR组)、帕瑞昔布低剂量组(L组,2?mg/kg)、帕瑞昔布中剂量组(M组,10?mg/kg)及帕瑞昔布高剂量组(H组,20?mg/kg)。复制单侧肺IR模型,检测肺内丙二醛(MDA)和髓过氧化物酶(MPO)含量、肺组织湿重/干重,观察肺组织病理改变及凋亡情况,检测肺内HO-1表达。结果 与C组比较,其他4组肺内MDA和MPO含量、肺组织湿重/干重、肺内凋亡细胞均增加(P?<0.05),IR组增加最多,L组次之,M组和H组增加最少,且M组和H组差异无统计学意义(P?>0.05)。肺IR后,IR组肺损伤最严重,不同剂量帕瑞昔布处理后,肺损伤均减轻,M组和H组较L组减轻更明显。C组内HO-1表达低,其余4组均升高(P?<
0.05),其中L组较IR组升高,M组和H组较L组进一步升高,M组和H组差异无统计学意义(P?>0.05)。结论 帕瑞昔布可减轻肺IR损伤,在一定程度呈剂量依赖性,其机制可能与增加肺组织中HO-1表达有关。
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关 键 词: | 再灌注损伤 细胞凋亡 帕瑞昔布 血红色加氧酶-1 |
收稿时间: | 2018/11/3 0:00:00 |
Effect of Parecoxib on heme oxygenase-1 and apoptosis after lung ischemia/reperfusion in rats* |
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Abstract: | Objective To explore the effect of different doses of Parecoxib on expression of HO-1 and apoptosis in rat model of lung ischemia-reperfusion (IR). Methods Totally 40 male adult Sprague-Dawley rats were randomly divided into 5 groups: control group (group C), ischemia reperfusion group (group I/R), Parecoxib group (2?mg/kg, group L), parecoxib group (10?mg/kg, group M), and parecoxib group (20?mg/kg, group H). Pulmonary ischemia-reperfusion injury model was established by blocking the left hilum of lungs. The wet/dry weight ratio (W/D), MDA and MPO of lung tissues were measured. Apoptosis, histological morphology of lung tissue as well as the expression of HO-1 in lung were detected. Results MDA, MPO, W/D and apoptosis rate were increased significantly in group I/R when compared with those in normal group, which was attenuated by treatment of Parecoxib (P?0.05). There was no significant difference in MDA, MPO, W/D and apoptosis rate between group M and group H (P?>?0.05). Pulmonary injury was the most severe in group I/R, which was attenuated by different doses of Parecoxib in dose dependent manner. HO-1 was only slightly expressed in group C, while significant upregulation of HO -1 was detected in group I/R (P?0.05) and was further elevated with treatment of Parecoxib. There was no statistical significant difference in HO -1 between group M and H (P?>?0.05) Conclusions Parecoxib attenuates lung ischemia-reperfusion injury dose-dependently probably through increased expression of HO-1. |
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Keywords: | reperfusion injury apoptosis Parecoxib heme oxygenase-1 |
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