| LncRNA-ATB 在非小细胞肺癌细胞中的作用及其机制探讨 |
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| 引用本文： | 李柏森,姜鹤群,文敏,易红梅,李涛. LncRNA-ATB 在非小细胞肺癌细胞中的作用及其机制探讨[J]. 中国现代医学杂志, 2019, 29(2): 23-28 |
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| 作者姓名： | 李柏森 姜鹤群 文敏 易红梅 李涛 |
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| 作者单位： | [1. 西南医科大学附属医院 肿瘤科，四川 泸州 646099 ；2. 成都医学院第一附属医院 肿瘤科，四川 成都 610500 ；3. 电子科技大学医学院附属肿瘤医院( 四川省癌症防治中心)，四川成都 610041 ；4. 成都医学院第一附属医院 胸心外科，四川 成都 610500] |
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| 基金项目： | 四川省教育厅重点项目（No ：13ZA0207）；四川省科技计划项目（No ：2017JY0307） |
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| 摘 要： | 目的 检测被转化因子激活的长链非编码RNA（LncRNA-ATB）在非小细胞肺癌（NSCLC）细胞中的表达，及沉默LncRNA-ATB 对NSCLC 细胞侵袭和转移能力的影响。方法 采用实时荧光定量聚合酶链反应（qRT-PCR）检测NSCLC 细胞和正常支气管上皮细胞（HBEC）中LncRNA-ATB 的表达水平；采用细胞划痕实验和Transwell 实验检测沉默LncRNA-ATB 对NSCLC 细胞侵袭和转移的影响；采用荧光素酶报告基因实验和qRT-PCR 检测LncRNA-ATB 与microRNA-141（miR-141）的调控关系。结果 LncRNA-ATB 在NSCLC 细胞中的表达高于HBEC 细胞（P <0.05）。沉默NSCLC 中LncRNAATB表达后，细胞侵袭和转移能力降低。LncRNA-ATB 可竞争性结合miR-141，并下调其表达。结论LncRNA-ATB 在NSCLC 中表达上调，并可通过竞争性结合miR-141，促进NSCLC 的侵袭和转移。
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| 关 键 词： | 癌，非小细胞肺；上皮细胞；长链非编码RNA/RNA ；肿瘤浸润；肿瘤转移 |
| 收稿时间： | 2018-08-01 |
LncRNA-ATB promotes migration and invasion of NSCLC cellsby competitive binding with miR-141 |
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| Bai-sen Li,He-qun Jiang,Min Wen,Hong-mei Yi,Tao Li. LncRNA-ATB promotes migration and invasion of NSCLC cellsby competitive binding with miR-141[J]. China Journal of Modern Medicine, 2019, 29(2): 23-28 |
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| Authors: | Bai-sen Li He-qun Jiang Min Wen Hong-mei Yi Tao Li |
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| Affiliation: | [1. Department of Oncology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099,China; 2. Department of Oncology, the First Affiliated Hospital of Chengdu Medical College, Chengdu,Sichuan 610500, China; 3. Affiliated Cancer Hospital, School of Medicine, University of Electronic Scienceand Technology of China (Sichuan Cancer Prevention and Control Center), Chengdu, Sichuan 610041,China; 4. Department of Thoracic and Cardiac Surgery, the First Affiliated Hospital ofChengdu Medical College, Chengdu, Sichuan 610500, China] |
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| Abstract: | Objective To explore the expression of LncRNA-ATB in non-small cell lung cancer (NSCLC),and investigate the effect and underlying mechanism of LncRNA-ATB on migration and invasion of NSCLCcells. Methods Expression of LncRNA-ATB in NSCLC cells and normal bronchial epithelial cells was analyzedby qRT-PCR. Effect of LncRNA-ATB on migration and invasion of NSCLC cells was detected by Transwellassay and wound healing assay. Luciferase assay was performed to detect whether LncRNA-ATB could sequestermiR-141. qRT-PCR was performed to detect the relationship between LncRNA-ATB and miR-141 in NSCLCcells. Results LncRNA-ATB was up-regulated in the NSCLC cells compared to the normal bronchial epithelial cells(P < 0.05). Migration and invasion of NSCLC cells were suppressed after silencing LncRNA-ATB. LncRNA-ATB could directly bind to miR-141 and down-regulate its expression in NSCLC cells. Conclusions LncRNA-ATB is upregulatedin NSCLC cells, and could promote migration and invasion of NSCLC cells by competitive binding to miR-141. |
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| Keywords: | carcinoma, non-small-cell lung epithelial cells RNA, long noncoding neoplasm invasiveness neoplasm metastasis |
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