骨关节炎滑膜中尿激酶型纤溶酶原激活物及其受体蛋白的表达

目的 :检测尿激酶型纤溶酶原激活物 (uPA)及其受体蛋白 (uPAR)在骨关节炎 (OA)滑膜中的表达 ,探讨uPA系统在OA发病中的病理作用。方法 :采用免疫组织化学方法检测 2 6例OA和 10例正常滑膜组织中uPA和uPAR蛋白的表达水平。结果 :2 6例OA滑膜组织中uPA阳性表达 19例 (73% ) ,uPAR阳性表达 14例 (5 4 6 % )。 10例正常滑膜组织中 ,uPA阳性表达 2例 (2 0 % ) ,uPAR阳性表达 1例 (10 % )。uPA和uPAR在OA滑膜中主要分布于滑膜衬里层细胞、衬里下层单核细胞、巨噬样细胞及血管内皮细胞。OA滑膜组织中uPA和uPAR蛋白的表达水平呈直线相关 (r =0 .92 0 ,P <0 .0 1)。结论 :OA滑膜组织中高水平的uPA和uPAR蛋白表达 ,提示在OA滑膜炎症和软骨降解等病理过程中 ,uPA系统起着重要作用 ,uPA起协同作用

Expression of urokinase-type plasminogen activator and its receptor protein in synovial tissues from osteoarthritis

Objective To study the expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in the synovial tissures, and the significance of uPA and uPAR in degradation of extracellular matrices in osteoarthritis (OA), then analyse the possible relationship between uPA and uPAR. Methods Immunohistochemical analysis technique was used to detect uPA and uPAR protein expression and distribution in synovial tissues in 26 OA patients and 10 mormal individuals. Results Positive staining of uPA and uPAR protein were detected in 19 cases in the 26 OA samples (73%), while only 2 positive cases were seen in the 10 mormal tissues (20%). The expression rate of uPA protein was markedly higher in OA than in the normal (P<0.01). The expression of uPAR was seen in 14 cases in all 26 OA samples (54.6%), while there was only one case in 10 normal samples (10%), the positive rate was significantly higher in OA than in normal samples (P<0.05). Positive expression of uPA and uPAR proteins were found in synovial lining cells, mononuclear cells, macrophage-like cells and endothelial cells. Using the correction analysis, we found a positive correlation between uPA and uPAR reactivity in the synovial tissues in OA (r=0.920, P<0.01). Conclusions The high expression of uPA and uPAR protein in OA synovial tissues indicate that the uPA system may play an important role in the process of synovitis and degradation of OA cartilage extracellular matrices. In the pathologic process of OA, uPA and uPAR coordinate each other, lead to the genesis and development of OA cartilage degradation.