骨桥蛋白和p21蛋白在结直肠癌中表达的相关意义

目的:探讨骨桥蛋白(OPN)和p21在原发性结直肠癌组织中的表达及转移中的作用。方法:采用免疫组化SP法检测60例结直肠癌组织中OPN和p21的表达情况。结果:OPN和p21的阳性表达主要定位于癌细胞的细胞浆;OPN和p21在60例结直肠癌组织中阳性表达率分别为66.7%(40/60)和61.7%(37/60)。在发生转移的结直肠癌组织中OPN和p21阳性表达率分别为83.3%(25/30)和80.0%(24/30),高于无转移的结直肠癌组织中OPN(50.0%,15/30)和p21(43.3%,13/30)的阳性表达率,差异具有显著性,(P<0.05)。两者均阳性表达的33例结直肠癌有24例(72.7%)已发生转移,与两者均阴性表达的8例结直肠癌仅有1例(12.5%)发生转移相比较,差异有显著性,(P<0.05),提示OPN和p21均阳性表达与肿瘤转移的发生密切相关。OPN和p21的表达与肿瘤的大小、肿瘤的分化、分期、性别、年龄等因素无关,但与肿瘤侵袭的发生有关,(P<0.05)。OPN和p21的表达成显著正相关(r=0.286,P<0.05)。结论:OPN和p21的表达与结直肠癌侵袭转移的发生密切关系,两者均阳性表达时对预测结直肠癌已发生转移具有指导意义。

Expression of osteopontin and p21 in primary colorectal cancer and their clinical significance

Objective:To investigate the expression of osteopontin(OPN) and p21 in primary colorectal cancer(PCC) tissue.Methods:Expressions of OPN and p21 in primary colorectal cancer tissues from 60 patients with primary colorectal cancer were detected using SP immunohistochemical technique.Results:OPN and p21 expression was mainly located in the cytoplasm of PCC cell.The positive rates of OPN and p21 in primarycolorectal cancer tissues were 66.7% and 61.7%,respectively.The positive rates of OPN and p21 in primary colorectal cancer tissues with metastasis were 80.0% and 73.3%,respectively,which were higher than that in primary colorectal cancer tissues without metastasis.OPN and p21 expressions were remarkably correlated with the metastasis in PCC(P<0.05).Cases both OPN and p21 erpressed in primary colorectal cancer tissue were 33,out of them,24 cases had metastasis,however,in the cases both OPN and p21 negative expressed,only 1 case had metastasis.Both OPN and p21 expression was remarkably related with the metastasis in PCC(P<0.05).The positive rates of OPN and p21 weren not related to the size of tumor,gender,age and stage of tumor,while they were correlated with metastasis of tumor.Conclusion:Both OPN and p21 are significantly related to the metastasis of PCC and over expression of both OPN and p21 has some predicting significance of metastasis for primary colorectal cancer.