Thymidine phosphorylase is regulated by tamoxifen in T47D breast cancer cell line |
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Authors: | Utsunomiya Hirotoshi Ueshima Hirokazu Kawashiro Mari Yang Qifeng Nakamura Misa Nakamura Yasushi Sakurai Takeo Mori Ichiro Kakudo Kennichi |
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Affiliation: | Hirotoshi Utsunomiya, Clinical and Basic Research Institute of Molecular Cellular Biology and Bioengineering, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, Japan. utsu@wakayama-med.ac.jp |
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Abstract: | BACKGROUND: Thymidine phosphorylase (TP) has diverse functions within cells, including increasing the sensitivity of cancer cells to cytotoxic drugs including 5-fluorouracil (5-FU) and methotrexate. However, the regulators of TP still remains largely unknown. METHOD: In this study, we examined whether tamoxifen has specific effects on TP expression in the T47D breast cancer cell line. We studied the TP expression in the T47D cell line before and after tamoxifen treatment using Western blot analysis and ELISA. RESULTS: We found that TP expression is significantly up-regulated in tamoxifen-treated cells compared with control. CONCLUSIONS: Our findings suggest that tamoxifen may increase chemotherapy sensitivity through TP up-regulation for treatment regimens including doxifluridine, capecitabine, and/or methotrexate. |
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Keywords: | Breast cancer Thymidine phosphorylase Tamoxifen ELISA |
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