气体信号分子NO和CRP在脑血管性疾病及血管性痴呆患者血浆中的变化和意义

目的探讨血管性痴呆、脑血管疾病患者血浆中一氧化氮(NO)和C反应蛋白(CRP)水平变化及可能的临床意义。方法 1.收集脑血管疾病患者40例、血管性痴呆患者46例,正常对照50例。全部研究对象均应用简明精神状态量表、日常生活能力量表、Hachinski缺血指数和Hamilton抑郁量表量表进行检测,血管性痴呆患者用全面衰退量表分级。取空腹静脉血2ml,迅速分离血浆并置于-70℃冰箱保存。2.NO和CRP分别以硝酸还原酶法及散射比浊法进行测定。结果 1.NO水平:血管性痴呆组为39.84±17.28μmol/L、脑血管疾病组为42.72±17.19μmol/L,均较对照组的62.92±17.58μmol/L明显降低(P0.01);病例组之间差异无统计学意义(P0.05)。相关分析显示血浆NO水平与血管性痴呆患者痴呆的严重程度没有相关性。2.CRP水平:血管性痴呆组(5.26±3.47mg/L)和脑血管疾病组(3.01±1.78mg/L)较对照组(1.36±1.33mg/L)升高(P0.05),且血管性痴呆组血浆CRP水平明显高于脑血管疾病组(P0.05)。血浆CRP水平与血管性痴呆的严重程度呈正相关。3.各组内NO与CRP的相关分析显示:各组内NO与CRP血浆水平没有明显的相关性。结论 1.气体信号分子NO在血管性痴呆及脑血管疾病患者中水平降低,但与血管性痴呆患者痴呆的严重程度没有相关性;2.血管性痴呆和脑血管疾病患者CRP升高提示炎症可能参与了脑卒中的发病。

The Gasotransmitter NO and CRP in Cerebrovascular Diseases and Vascular Dementia

Objective To clarify the role of nitric oxide（NO） and C-reactive protein（CRP） in the pathogenesis of cerebrovascular diseases（CVD） and vascular dementia（VD）,we measured the plasma concentrations of NO and CRP in 40 patients with CVD,46patients with VD,and 50 normal controls（NC）.Methods Patients with CVD or VD and 50 NC were recruited.Mini Mental State Examination,Activity of Daily Living Scale,Hachinski Ischemic Score and Hamilton＇s depression scale were performed in all patients and controls.The severity of VD was evaluated according to Global Deterioration Scale.The plasma levels of NO and CRP were measured in all the objects by Nitrate/Nitrite colorimetric assay and immunoturbidimetry respectively.Results ① the plasma level of NO was significantly lower in the patients with VD（39.84±17.28μmol/L）,or with CVD（42.72±17.19μmol/L） than in the normal controls（62.92±17.58μmol/L）（P 0.01）.② The plasma level of CRP was higher in the cases with VD（5.26±3.47mg/l） and CVD（3.01±1.78mg/l） than in the normal controls（1.36±1.33mg/l）.The spearman correlation analysis showed that the severity of VD was positive correlation with CRP.③ No relationship was found between NO and CRP in NC,VD and CVD patients.Conclusions ① Gasotransmitter NO is decreased in patients withVD and CVD.② the Increase of CRP,a blood in？ ammatory marker,in patients with CVD and VD suggests the involvement of in？ ammatory in the pathogenesis of CVD.