Radioautographic investigation of monoaminergic neurons: An evaluation

This review outlines the most relevant contributions currently available on the detection and ultrastructural characterization of monoaminergic neurons by radioautography after administration of radiolabeled monoamines. It includes methodological considerations and then a critical analysis of the diagnostic value of the radioautographic method for catecholaminergic and serotoninergic neurons, emphasizing in particular its recent applications to the visualization of dopaminergic axon terminals. An attempt is then made to evaluate the method in terms of specificity, sensitivity and resolution and its possibilities with regard to quantitative analysis. Lastly, its value for approaching the dynamic and metabolic properties of monoaminergic neurons is stressed.