兔下颌骨骨折对颞下颌关节影响的实验研究

目的 观察下颌骨骨折对两侧颞下颌关节的影响，探讨损伤后颞下颌关节修复的机制。方法 采用新西兰大耳白兔30只。随机分为术后48h和1、3、5、8周5个时相组及一个对照组。每组5只，用多功能打击装王造成各组动物左侧下颌骨骨折。夹板坚固内固定骨折断，按时项处死动物取左侧颞下颌关节，观察伤侧颞下颌关节的组织学变化。用免疫组化方法检测髁状突软骨内VEGF及其受体Flt1的表达。结果 各时项标本均可见到颞下颌髁状突及关节盘不同程度的损伤，髁状突肥大细胞检测到VEGF及其受体Flt1的表达。结论 下颌骨骨折可致颞下颌关节损伤。VEGF和其受体Flt1可能参与颞下颌关节损伤的修复。

The empirical study on the effect of mandibular fracture on temporomandibular joint

Objective To observe the effect of mandibular fracture on bilateral temporomandibular joints. And research the mechanism of injuried TMJ repairing. Methods The fracture modds were made in the middle of the left mandible of the rabbits with muhifunctional striking equipment, then the specimens from the left lateral temporomandibular joints were taken after 48 hours and 1,3,5,8 weeks respectively. The expression of VEGF and its receptor at the fracture site was detected through immunohistochemistry and in situ hybridization. Results Lesion of bilateral temporomandibular joints were seen in all groups. VEGF and Fltl receptor of VEGF were detected in pillar calls of condyloid process in experimental groups after fracture. Conclusion Mandibular fracture can induce lesion of temporomandibular joints. VEGF and Fltl maybe take part in healing after temporomandibular joints injury.