515抗瘤方剂诱导白血病HL60细胞凋亡的研究

目的：观察中药５１５抗瘤方剂对白血病ＨＬ－６０细胞的影响。方法：采用光镜、透射电镜观察形态及结构，流式细胞术测量凋亡率，原位末端标记检测ＤＮＡ断裂。结果：细胞与药物共同孵育一定时间后出现固缩、核 碎裂等典型的凋亡形态学变化，药物浓度在０．０１３～０．０４g/ml之间，药物组与对照组凋亡率有显著性差异（Ｐ〈０．０１）。结论：５１５抗瘤方剂能诱导ＨＬ－６０细胞发生凋亡，细胞凋亡率随时间延长增加，且呈现剂量

Study on 515 Anti Tumor Recipe in Inducing Leukemic HL-60 Cells Apoptosis

OBJECTIVE: To observe the efficacy of 515 anti-tumor recipe on inducing human myeloid leukemic HL-60 cells apoptosis. METHODS: The morphological change of apoptotic cells was observed by light microscopy and transmission electromicroscopy (TEM), and flow cytometry and TUNEL reaction to further confirm the apoptosis of HL-60 cells. RESULTS: Typical apoptosis appeared such as marginal nuclei, chromatin condensation and nuclear fragmentation could be seen by light microscopy and TEM. The drug concentration was between 0.013-0.04 g/ml. The apoptosis rate is 30% in 0.04 g/ml group at the 72nd hour, but it was only 0.8% in the control group at the same time (P < 0.01). CONCLUSIONS: The 515 anti-tumor recipe can induce apoptosis in HL-60 cells. The apoptosis rate increased as the time extended. There was an enhanced efficiency of apoptosis in a dose-dependent manner.