515抗瘤方剂诱导白血病HL60细胞凋亡的研究 |
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引用本文: | 谢新生,高聆,宋玉琴,王春霞,赵家军,徐功立.515抗瘤方剂诱导白血病HL60细胞凋亡的研究[J].中国中西医结合杂志,2000,20(2):129-131. |
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作者姓名: | 谢新生 高聆 宋玉琴 王春霞 赵家军 徐功立 |
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作者单位: | 山东省立医院!济南250021 |
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摘 要: | 目的:观察中药515抗瘤方剂对白血病HL-60细胞的影响。方法:采用光镜、透射电镜观察形态及结构,流式细胞术测量凋亡率,原位末端标记检测DNA断裂。结果:细胞与药物共同孵育一定时间后出现固缩、核 碎裂等典型的凋亡形态学变化,药物浓度在0.013~0.04g/ml之间,药物组与对照组凋亡率有显著性差异(P〈0.01)。结论:515抗瘤方剂能诱导HL-60细胞发生凋亡,细胞凋亡率随时间延长增加,且呈现剂量
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关 键 词: | 细胞凋亡 HL-60细胞 白血病 515抗瘤方剂 |
Study on 515 Anti Tumor Recipe in Inducing Leukemic HL-60 Cells Apoptosis |
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Authors: | XIE Xinsheng GAO Ling SONG Yuqin Shandong Provincial Hospital Jinan |
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Abstract: | OBJECTIVE: To observe the efficacy of 515 anti-tumor recipe on inducing human myeloid leukemic HL-60 cells apoptosis. METHODS: The morphological change of apoptotic cells was observed by light microscopy and transmission electromicroscopy (TEM), and flow cytometry and TUNEL reaction to further confirm the apoptosis of HL-60 cells. RESULTS: Typical apoptosis appeared such as marginal nuclei, chromatin condensation and nuclear fragmentation could be seen by light microscopy and TEM. The drug concentration was between 0.013-0.04 g/ml. The apoptosis rate is 30% in 0.04 g/ml group at the 72nd hour, but it was only 0.8% in the control group at the same time (P < 0.01). CONCLUSIONS: The 515 anti-tumor recipe can induce apoptosis in HL-60 cells. The apoptosis rate increased as the time extended. There was an enhanced efficiency of apoptosis in a dose-dependent manner. |
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Keywords: | Chinese herbal medicine apoptosis HL 60 cells |
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