iNOS和caspase 3在胚胎停育绒毛和蜕膜组织中的表达

目的：探讨胚胎停育绒毛组织和蜕膜组织中半胱氨酸天冬氨酸蛋白酶3（caspase 3）和诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）的表达和临床意义。方法：选择2013年3月-2014年5月就诊于武警后勤学院附属医院妇产科计划生育门诊的30例不明原因早期胚胎停育的患者（病例组）和30例正常早期妊娠妇女（对照组）为研究对象，采用逆转录聚合酶链反应（RT-PCR）方法检测2组绒毛组织和蜕膜组织中caspase 3和iNOS mRNA的表达水平，采用免疫印迹法检测2组绒毛组织和蜕膜组织中caspase 3蛋白的表达水平。结果：①病例组绒毛组织和蜕膜组织中caspase 3 mRNA、总的caspase 3蛋白和活化的caspase 3蛋白的表达水平均高于对照组（P＜0.01）。②病例组绒毛组织和蜕膜组织中iNOS mRNA表达水平低于对照组（P＜0.01）。结论：正常早孕和胚胎停育患者绒毛组织和蜕膜组织中均有iNOS和caspase 3的表达，iNOS和caspase 3可能参与凋亡的增加进而引起胚胎停育的发生。

Expressions of iNOS and Caspase 3 in Chorionic Tissues and Decidual Tissues of Patients with Embryonic Diapauses

Objective:To study the expression and the clinical importance of the iNOS and caspase 3 in the chorionic and decidual tissues of the patients whose embryos stop developing. Methods:30 cases of early unexplained embryonic diapauses patients (case group) and 30 normal early pregnant women (control group) for the study, chorionic and deciduas tissues caspase 3 and iNOS levels were tested by RT-PCR method, chorionic and decidual caspase 3 protein expression levels were tested by Western blot. Results:①In the chorionic and decidual tissues, caspase 3 mRNA, the total caspase 3 protein and caspase 3 fragment protein expression levels in the case group was significantly higher than the control group (P<0.01).②In the chorionic and decidual tissues, iNOS mRNA expression level in the case group was significantly lower than the control group (P<0.01). Conclusions:In the chorionic and decidual tissues,iNOS and caspase 3 positive expression in the normal pregnancy and in the patients whose embryos stop developing. The iNOS and caspase 3 may involve in apoptosis, and then cause embryo stopping development.