Stimulation of the regrowth of MPP+-damaged dopaminergic fibers by the treatment of mesencephalic cultures with basigin |
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| Authors: | Y Mitsumoto A Watanabe T Miyauchi F Jimma T Moriizumi |
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| Institution: | (1) Neurodegenerative Diseases Research Group, and, JP;(2) Psychiatric Research Group, Second Institute of New Drug Research, Otsuka Pharmaceutical Co., Ltd., Tokushima,, JP;(3) Japan Immunoresearch Laboratories Co., Ltd., Gunma, and, JP;(4) Department of Anatomy, School of Medicine, Shinshu University, Nagano, Japan, JP |
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| Abstract: | Summary. Basigin (Bsg) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and widely expressed in the central
nervous system. To elucidate functional role of Bsg in the central nervous system, the effects of its glutathione-S-transferase
(GST) fusion protein on the number and neurite outgrowth of cultured rat mesencephalic dopaminergic neurons were measured.
The fusion protein was not able to promote the survival and neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons
under serum-free condition. However, the treatment of 1-methyl-4-phenylpyridinium (MPP+)-exposed cultures with the fusion protein resulted in stimulation of the regrowth of damaged TH-positive fibers. Basic fibroblast
growth factor (bFGF) also stimulated the regrowth of neurites in damaged neurons. These results indicate that Bsg may play
an important role in the regrowth of damaged dopaminergic fibers.
Received May 9, 2001; accepted July 2, 2001 |
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| Keywords: | : Basigin immunoglobulin superfamily dopaminergic neurons culture regrowth MPP+ |
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