| 人类TAP2基因克隆及真核表达质粒的构建 |
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| 引用本文： | 王兰,李殿俊,吕雪莹,杨秋霞,刘玉.人类TAP2基因克隆及真核表达质粒的构建[J].哈尔滨医科大学学报,2004,38(1):5-8. |
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| 作者姓名： | 王兰 李殿俊 吕雪莹 杨秋霞 刘玉 |
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| 作者单位： | 哈尔滨医科大学,免疫学教研室,黑龙江,哈尔滨,150086;哈尔滨医科大学,免疫学教研室,黑龙江,哈尔滨,150086;哈尔滨医科大学,免疫学教研室,黑龙江,哈尔滨,150086;哈尔滨医科大学,免疫学教研室,黑龙江,哈尔滨,150086;哈尔滨医科大学,免疫学教研室,黑龙江,哈尔滨,150086 |
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| 基金项目： | HeilongjiangProvinceNaturalScienceFoundation(D0 12 2 ) |
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| 摘 要： | 目的 克隆抗原处理相关转运体亚基TAP2基因片段并构建真核表达载体。方法 从EB病毒刺激的人B淋巴母细胞系(B-LCL)中提取总RNA，用RT-PCR从中扩增出TAP2 cDNA。将TAP2 cDNA插入表达载体pcDNA3．1／VS-His-TOPO中，构建重组表达载体pcDNA3．1／V5-His-TOPO-TAP2，并进行测序分析。结果 经RT-PCR和测序鉴定，成功地克隆了人TAP2 cDNA。结论 获得了人TAP2 cDNA并构建了重组真核表达载体，对TAP2基因产物在体外表达、进一步研究TAP2分子在肿瘤基因治疗方面的作用具有重要的意义。
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| 关 键 词： | 抗原处理相关转运体-2 基因克隆 序列分析 真核表达 |
Cloning of human gene TAP2 and construction of its eukaryotic expression vector |
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| WANG Lan,LI Dian-jun,U Xue-ying,YANG Qiu-xia,LIU Yu.Cloning of human gene TAP2 and construction of its eukaryotic expression vector[J].Journal of Harbin Medical University,2004,38(1):5-8. |
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| Authors: | WANG Lan LI Dian-jun U Xue-ying YANG Qiu-xia LIU Yu |
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| Abstract: | Objective To clone the transporter associated with antigen processing 2 (TAP2) gene and construct its eukaryotic expression vector.Methods Human TAP2 cDNA was amplified by RT PCR from Epstein Barr virus (EBV) treated human B lymphoblastoid cell line (B LCL).The TAP2 cDNA was inserted into the expression plasmid pcDNA3.1/V5 His TOPO to construct the recombinant expression vector pcDNA3.1/V5 His TOPO TAP2.Results RT PCR and DNA sequencing showed that human TAP2 cDNA was cloned successfully.Conclusion Human TAP2 cDNA is obtained and the recombinant eukaryotic expression vector is constructed,which provide the possibility for further expression of TAP2 in vitro ,and lay the foundation for further studying the function of TAP2 protein in cancer gene therapy. |
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| Keywords: | TAP2 gene cloning sequencing eukaryotic expression |
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