高压氧对急性CO中毒大鼠小脑组织NO及NOS活性的影响

目的　研究高压氧(HBO)对急性一氧化碳(CO)中毒大鼠小脑组织中一氧化氮(NO)、一氧化氮合酶(NOS)活性的影响，探讨NO、NOS与急性CO中毒脑损伤的关系及HBO对NO、NOS的作用机制。方法　实验用Wistar大鼠80只，随机分为5组正常对照组(C)，CO染毒组(CO)，CO染毒+HBO1次治疗组(HBO1)，CO染毒+HBO3次治疗组(HBO

Effect of hyperbaric oxygen on nitric oxide and activities of nitric oxide synthase in cerebellum after carbon monoxide poisoning in the rat

Objective To study levels of nitric oxide (NO ) and nitric oxide synthase (NOS) in the rat cerebellum after exposure to carbon monox ide with or without HBO therapy. Methods Eighty male Wistar rats were randomly divided into 5 groups: control group,CO exposurewi without treatment,CO exposure with 1 HBO treatment,CO exposure with 3 HBO treatments,CO exposure with one normobaric oxygen (NBO) treatment NO levels were measured by Griess' method, and NOS activity was measured respectively by spectrophotometry. NO, NOS levels were com pared among the 5 groups. Results In the rat cerebellum , the level of NO increased and the level of NOS decreased after CO exposure. The level of NO decreased after HBO therapy, but not after NBO therapy. NBO and HBO therapy resulted in a return of NOS levels to baseline. Conclusions Activity of NOS was inhibited in the cerebell um after (CO) exposure. Both HBO and NBO therapies restored NOS activity. The production of NO increased in the cerebellum after CO exposure and only HBO (not NBO) restored NO production at the site. The demonstrated changes in levels of NO and NOS may play an important role in the central nerve system damage resulting from CO poisoning. NBO protected brain tissue after CO exposure in certain extent.