HPLC测定大黄廑虫丸中芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量

目的：建立HPLC同时测定大黄廑虫丸中芦荟大黄素、大黄酸，大黄素、大黄酚和大黄素甲醚含量的方法。方法：色谱柱为Shimadzu VP-ODS柱（4．6mm×250mm，5um），流动相为乙腈-0．1％磷酸梯度洗脱，流速为1．0mL·min-1，检测波长为254nm，柱温为30℃。结果：芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5种成分分别在0．042—0．840ug（r=0．9996）、0．168～3．352ug（r=0．9998）、0．084～1．680ug（r=0．9997）、0．093～1．852ug（r=0．9999）和0．174—3．488ug（r=0．9994）呈良好线性关系，平均回收率分别为98．87％（RSD=1．43％）、98．63％（RSD=0．64％）、97．80％（RSD=1．46％）、97．29％（RSD=0．97％）和98．45％（RSD=0．88％）。结论：本法简便、快速、准确，可用于大黄廑虫丸的质量控制。

Determination of Aloe-emodin, Rhein, Emodin, Chrysophanol and Physcion in Dahuang Zhechong Pills by HPLC

Objective： To develop a HPLC method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Dahuang Zhechong Pills. Methods： The sample was separated on a Shimadzu VP-ODS（4. 6 mm ×250 mm, 5 um） with acetonitrile-0. 1% phosphoric acid in gradient elution. The flow rate was 1.0 mL. min-1, the detection wavelength was 254 nm and the column temperature was 30 ℃. Results： Aloe-emodin, rhein, emodin, chrysophanol and physcion had good linear relationship in the range of 0. 042 × 0. 840 ug （r = 0. 999 6） ,0. 168 -3. 352 ug（r = 0. 999 8） ,0. 084 - 1. 680 ug（r =0. 999 7） ,0. 093 - 1. 852 ug（r =0. 999 9）and 0. 174 -3. 488 ug（r =0. 999 4）. Their average recoveries were 98. 87 % （RSD = 1.43 %, n =6）, 98. 63 % （RSD = 0.64 %, n=6）, 97.80 %（RSD=1.46 %, n=6）, 97.29 %（RSD=0.97 %, n=6）, and 98.45 %（RSD= 0. 88 %, n = 6）, respectively. Conclusion： The method is rapid, simple, accurate, and applicable for the quality control of Dahuang Zhechong pills.