Comparison of different PCR protocols for the detection and diagnosis of Plasmodium falciparum |
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| Authors: | N. Oster I. Z. Abdel-Aziz A. Stich B. Coulibaly B. Kouyatè K. T. Andrews J. E. McLean M. Lanzer |
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| Affiliation: | (1) Hygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany;(2) Present address: Schmiedegasse 2, 79730 Murg, Germany;(3) Present address: Department of Parasitology, Faculty of Medicine, Cairo University, Hani El-Lobody Str. 11 Omrania Sharkia, El-Haram, 12111 Giza, Egypt;(4) Present address: Missionsärztliches Institut, AG Tropenmedizin und Seuchenbekämpfung, Hermann-Schell-Str. 7, 97074 Wurzburg, Germany;(5) Centre de Recherche en Santéà Nouna, , BP02 Nouna, Burkina Faso;(6) Present address: Clinical Tropical Medicine Laboratory, The Queensland Institute of Medical Research, PO Box Royal Brisbane Hospital, Herston, 4029 Queensland, Australia |
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| Abstract: | An assessment of differing PCR protocols for the diagnosis of Plasmodium falciparum infection was performed on samples from an area of holoendemic malaria transmission in western Burkina Faso. The PCR protocols had generally high sensitivities (>92%) and specificities (>69%), but the negative predictive values (NPV) were moderate and differed widely among the PCR protocols tested. These PCR protocols that amplified either the P. falciparum pfcrt gene or the small subunit ribosomal DNA were the most reliable diagnostic tools. However, the moderate NPV imply that more than one PCR protocol should be used for diagnosis in holoendemic areas.N. Oster and I.Z. Abdel-Aziz have contributed equally to this work. |
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