快速筛选金钱松内生真菌抗真菌活性的研究

目的：筛选金钱松内生真菌抗病源真菌的活性菌株。方法：首次对金钱松内生真菌进行了分离,以稻瘟霉PyricuLaria oryza P-2b为筛选模型,采用微量稀释法筛选金钱松内生真菌抗真菌活性,研究其抗病源真菌的能力。结果：金钱松内存在着大量且具有抗稻瘟霉P-2b活性的内生真菌,占内生真菌总数的44.8％。其中JJ314,JJ323可诱导稻瘟霉P-2b菌丝念珠状、膨大变形,JJ324可抑制孢子萌发,它们对红色毛癣菌Trichophyton rubrum、新型隐球菌Cryptococcus neoformans、白色念珠菌Candida albicans等病源真菌都具有强烈的活性。结论：金钱松内生真菌是潜在的抗真菌药物资源。

Study on fast screening antifungus activity of endophytes from Pseudolarix kaempferi

Objective: To screen antifungal activity of endophytes from Pseudolarix kaempferi. Method: Endophytes from P.kaempferi were separated.By means of microdilution method,antifungal active endophytes were fast screened by Pyricularia oryzae P-2b model,and activity of endophytes against pathogenic fungus was studied.Result: 44.8% of endophytes showed activity against P.oryzae P-2b in Pseudolarix kaempferi.Among them JJ314,JJ323 introduced formation of characteristic beads and swellings on the growing hyphae,JJ324 inhibited the conidia germination.They all showed activity against Trichophyton rubrum,Cryptococcus neoformans and Candida albicans.Conclusion: Endophytes from P.kaempferi are a potential resource for the development of antifungal agent.