lncRNA ANRIL和VEGF在原因不明复发性流产绒毛中表达

目的:探讨长链非编码RNA(LncRNA)细胞周期激酶抑制因子4基因座中反义非编码RNA(ANRIL)和血管内皮生长因子(VEGF)在原因不明复发性流产(URSA)患者绒毛中的表达。方法:选取2017年8月-2019年6月在本院就诊的URSA患者62例(观察组),同期行人工流产术的正常早孕者62例(对照组)。观察组行清宫手术、对照组行人工流产术时采集新鲜胎盘绒毛组织,实时荧光定量PCR(qRT-PCR)法检测绒毛组织中lncRNA ANRIL、VEGF mRNA表达水平;Pearson法分析URSA患者绒毛组织中lncRNA ANRIL和VEGF mRNA表达水平的相关性;采用多因素logistic回归分析影响URSA发生的因素。结果:观察组绒毛组织中lncRNA ANRIL(0.74±0.20)、VEGF mRNA(0.30±0.09)表达水平均低于对照组(1.00±0.25、1.00±0.18)(P<0.05),lncRNA ANRIL与VEGF mRNA表达水平呈显著正相关(r=0.484,P<0.05),lncRNA ANRIL、VEGF mRNA表达水平均为影响URSA发生的保护因素(P<0.05)。结论:URSA患者绒毛组织中lncRNA ANRIL和VEGF表达水平均显著下调且密切相关,可能共同影响疾病发生。

The expression of lncRNA ANRIL and VEGF in villi of women with unexplained recurrent spontaneous abortion

Objective:To investigate the expression of antisense non-coding RNA in the long non-coding RNA(lncRNA)INK4 locus(ANRIL)and vascular endothelial growth factor(VEGF)in villi of women with unexplained recurrent spontaneous abortion(URSA).Methods:62 women with URSA were selected in observation group from August 2017 to June 2019,and 62 health women with abortion because of unwanted pregnancy were selected in control group.Fresh placental villi of women in the two groups were collected,and the expression levels of lncRNA ANRIL and VEGF mRNA in villi were detected by real-time fluorescence quantitative PCR(qRT-PCR).Pearson method was used to analyze the correlation between the lncRNA ANRIL level and the VEGF mRNA level in the villi.Multivariate logistic regression analysis was used to analyze the influencing factors of the occurrence of URSA.Results:The expression levels of lncRNA ANRIL(0.74±0.20)and VEGF mRNA(0.30±0.09)in the villi of women in the observation group were all significant lower than those(1.00±0.25,1.00±0.18)of women in the control group(P<0.05).LncRNA ANRIL level was significantly positively correlated with VEGF mRNA level(r=0.484,P<0.05).The lncRNA ANRIL and VEGF mRNA expression levels were protective factors affecting the occurrence of URSA(P<0.05).Conclusion:The down-regulated of expression levels of lncRNA ANRIL and VEGF in the villi of women with URSA is closely related each other significantly,which may jointly affect the occurrence of URSA.