RND1通过p53蛋白促进PC12细胞凋亡

目的 探讨RND1对神经细胞凋亡的作用及可能的相关机制。方法 利用脂质体转染质粒,建立体外高表达RND1细胞模型,具体分组为flag-RND1组和flag组; 以流式细胞术、Q-PCR和免疫印迹等方法检测各组细胞内RND1、p53、cleaved-Caspase3蛋白表达及凋亡水平。结果 与对照组比较,flag-RND1组RND1表达水平升高,流式细胞术显示flag-RND1组细胞凋亡水平增高,同时flag-RND1组细胞内凋亡蛋白cleaved-Caspase3的表达水平较flag组升高(P<0.05); 伴随凋亡情况改变,p53蛋白表达水平降低。结论 小GTP酶蛋白家族成员RND1可能通过p53蛋白参与调节神经细胞凋亡,这可能是1个新的神经元凋亡调控位点。

RhoE regulated neurocytes apoptosis in PC12 cells through p53 protein

ObjectiveTo explore the mechanism of RND1 which belongs to small GTPase family regulated neurocytes apoptosis.Methods The plasmid fragment to PC12 cells with liposomes was transfected to build over-expression model. The test groups were performed as follows: flag-RND1group, flag-group. Flow cytometry, Q-PCR and western blot were performed to test the expressive levels of mRNA, protein, and cell apoptosis.Results The mRNA and protein level of RND1 increased in flag-RND1 group,so dose cleaved-Caspase3,flow cytometry also showed the apoptotic rate increased(P<0.05). Meanwhile, the protein expressive level of p53 decreased.Conclusion RND1, a member of the small GTPase protein family might regulate of neuronal apoptosis through p53 protein, which might be a new neuronal apoptosis regulatory site.