银杏叶提取物对脑动脉粥样硬化大鼠血清TG、TC和HO-1水平的影响

目的 探讨银杏叶提取物对脑动脉粥样硬化大鼠血清甘油三酯(Triglyceride,TG)、总胆固醇(Total cholesterol,TC)和血红素氧合酶-1(Heme oxygenase-1,HO-1)水平的影响。方法 30只SD健康雄性大鼠,20只大鼠建立老龄脑动脉粥样硬化模型,分为假手术组、模型组和银杏叶组; 检测血清TG、TC、高密度脂蛋白胆固醇(High density lipoprotein cholesterol,HDL-C)、HO-1水平、动脉硬化指数(Arteriosclerosis index,AI); 采用MTT检测细胞增殖能力,流式细胞仪检测脑动脉平滑肌细胞凋亡,Western blot法检测Caspase-3、Bcl-2、Bax蛋白水平。结果 与模型组比较,银杏叶组脑动脉斑块面积、新生内膜面积、内膜厚度降低,管腔面积升高(P<0.05); 与模型组比较,银杏叶组TG、HDL-C水平、AI降低,TC、HO-1水平升高(P<0.05); 与模型组比较,银杏叶组24、48、72 h细胞凋亡率降低(P<0.05),银杏叶组24 h细胞凋亡率低于48和72 h细胞凋亡率(P<0.05); 与模型组比较,银杏叶组48、72 h细胞增殖率升高(P<0.05),银杏叶组24h细胞增殖率低于48和72 h细胞增殖率(P<0.05); 与模型组比较,银杏叶组Caspase-3、Bax水平降低,Bcl-2水平升高(P<0.05)。结论 银杏叶提取物能够改善脑动脉粥样硬化大鼠血清TG、TC、HO-1水平,并通过下调Caspase-3、Bax蛋白表达,上调Bcl-2表达来促进脑动脉平滑肌细胞增殖,抑制脑动脉平滑肌细胞凋亡。

The effects of Ginkgo biloba extract on serum TG,TC and HO-1 levels in rats with cerebral atherosclerosis

ObjectiveTo investigate the effects of Ginkgo biloba extract on serum triglyceride(TG), total cholesterol(TC)and heme oxygenase-1(HO-1)levels in rats with cerebral atherosclerosis.Methods Thirty aged healthy male SD rats were collected aand divided into sham operation group, model group and Ginkgo biloba group. Serum TG, TC, high density lipoprotein cholesterol(HDL-C), HO-1 levels and arteriosclerosis index(AI)were measured. MTT was used to detect cell proliferation, flow cytometry was used to detect apoptosis of cerebral vascular smooth muscle cells, and Western blot was used to detect Caspase-3, Bcl-2 and Bax protein levels.Results Compared with model group, the area of cerebrovascular plaque, neointima, intima thickness decreased, lumen area increased inGinkgo biloba group(P<0.05). Compared with model group,the levels of TG, HDL-C and AI decreased, while the levels of TC and HO-1 increased in Ginkgo biloba group(P<0.05). Compared with model group,the apoptotic rate of Ginkgo biloba group decreased at 24, 48 and 72 h(P<0.05). The apoptotic rate at 24h in Ginkgo biloba group was lower than that at 48 and 72 h(P<0.05). Compared with model group,the cell proliferation rate of Ginkgo biloba group increased at 48 and 72 h(P<0.05),the cell proliferation rate of Ginkgo biloba group at 24 h was lower than that at 48 and 72 h(P<0.05). Compared with model group,the levels of Caspase-3 and Bax decreased and the level of Bcl-2 increased in Ginkgo biloba group(P<0.05).Conclusion Ginkgo biloba extract could improve the levels of serum TG, TC and HO-1 in rats with cerebral atherosclerosis. It could promote the proliferation of cerebral vascular smooth muscle cells and inhibit the apoptosis of cerebral vascular smooth muscle cells by down-regulating Caspase-3 and Bax protein levels and up-regulating Bcl-2 level.