蛋白激酶D1在宫颈癌中的表达及临床意义

目的:探究蛋白激酶D1(PKD1)在宫颈癌组织中的表达及临床意义。方法:收集2018年1月-2019年12月本院48例宫颈癌(宫颈癌组)、42例高级别宫颈上皮内瘤变(CINⅢ组)及38例正常宫颈者(对照组)宫颈组织标本,检测标本PKD1表达,分析其与宫颈癌临床病理特征及增殖细胞核抗原(Ki67)表达相关性。结果:3组宫颈标本PKD1阳性表达率及免疫组化评分均为宫颈癌组(81.3%、4.7±0.8分)>CINⅢ组(61.9%、3.1±0.6分)>对照组(7.9%、0.9±0.3分)(P<0.05)。宫颈癌组中,PKD1阳性39例、PKD1阴性9例,PKD1阳性组FIGO分期、低分化、淋巴结转移及Ki67表达阳性率均高于PKD1阴性组(P<0.05)。经Spearman秩相关检验,宫颈癌组织PKD1免疫组化评分与FIGO分期、淋巴结转移及Ki67免疫组化评分呈显著正相关(r=0.439、0.411、0.517,P<0.05),而与肿瘤分化程度呈显著负相关(r=-0.304,P<0.05)。结论:PKD1在宫颈癌组织中高表达,与宫颈癌的发生发展有关,可作为宫颈癌诊疗及预后评估的重要指标。

Expression of protein kinase D1 in cervical cancer tissue and its clinical significance

Objective:To investigate the expression of protein kinase D1(PKD1)in cervical cancer tissue,and to study its clinical significance.Methods:From January 2018 to December 2019,48 specimens of cervical tissue of 48 patients with cervical cancer(in group A),42 specimens of cervical tissue of patients with high-grade cervical intraepithelial neoplasia(CINⅢ)(in group B),and 38 specimens of cervical tissue of patients with normal cervix(in group C)were collected.Immunohistochemical method was used to detect the expression of PKD1 in cervical tissue of the three groups.The correlation between the expression of PKD1 and the clinicopathological characteristics of cervical cancer or the expression of proliferating cell nuclear antigen(such as Ki67)was analyzed.Results:The positive expression rate and immunohistochemical score of PKD1 in cervical tissue samples in group A(81.3%,4.7±0.8 points)were the highest,followed by those(61.9%,3.1±0.6 points)in group B,and those(7.9%,0.9±0.3 points)in group C were the lowest(P<0.05).In group A,there were 39 patients with PKD1 positive and 9 patients with PKD1 negative.The FIGO stage,and the rates of low differentiation,lymph node metastasis,and Ki67 expression positive of patients with PKD1 positive were significant higher than those of patients with PKD1 negative(P<0.05).Spearman rank correlation test showed that PKD1 immunohistochemical score was significantly positively correlated with the FIGO stage,lymph node metastasis situation,and Ki67 immunohistochemical score of patients with cervical cancer(r=0.439,0.411,0.517,P<0.05),but was significantly negatively correlated with their tumor differentiation degree(r=-0.304,P<0.05).Conclusion:PKD1 is highly expressed in cervical cancer tissue,which is closely related to the occurrence and development of cervical cancer.So it may be used as an important indicator for evaluating the values of diagnosis,treatment and prognosis of cervical cancer.