| 小鼠BPI N端功能片段(muBPI_(25)蛋白)体外杀菌和中和内毒素作用研究 |
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| 引用本文： | 吕喆,王炜,范宜强,刘振龙,孔庆利,温铭杰,龙军,李晨,许晴,安云庆.小鼠BPI N端功能片段(muBPI_(25)蛋白)体外杀菌和中和内毒素作用研究[J].中国免疫学杂志,2010,26(4). |
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| 作者姓名： | 吕喆 王炜 范宜强 刘振龙 孔庆利 温铭杰 龙军 李晨 许晴 安云庆 |
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| 作者单位： | 首都医科大学基础医学院免疫学系,北京,100069 |
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| 摘 要： | 目的:建立胞内杀菌和体外中和内毒素实验模型,检测muBPI25目的蛋白对胞内寄生菌的抑杀作用和对内毒素的中和作用。方法:将pcDNA3.1(+)-muBPI36-259质粒导入RAW264.7细胞,用胞内寄生G+/G-菌感染上述细胞建立muBPI25目的蛋白胞内杀菌实验模型;将pSecTag2B-muBPI36-259与双荧光素酶报告基因质粒共转染RAW264.7细胞,建立体外检测muBPI25蛋白中和内毒素实验模型。结果:胞内杀菌实验模型证实muBPI25目的蛋白对G-伤寒杆菌具有抑杀作用;中和内毒素实验模型证实muBPI25目的蛋白对内毒素具有中和作用。结论:首次证实小鼠BPI N端功能片段,即muBPI25目的蛋白对G-菌具有抑杀作用,对其裂解产物内毒素具有中和作用。
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| 关 键 词： | 杀菌/渗透性增强蛋白 RAW264.7细胞 胞内寄生菌 内毒素 |
Studies on antibacterial activity and endotoxin neutralization of murine BPI N-terminal functional fragment(muBPI_(25) protein)in vitro |
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| L Zhe,WANG Wei,FAN Yi-Qiang,LIU Zhen-Long,KONG Qing-Li,WEN Ming-Jie,LONG Jun,LI Chen,XU Qing,AN Yun-Qing.Studies on antibacterial activity and endotoxin neutralization of murine BPI N-terminal functional fragment(muBPI_(25) protein)in vitro[J].Chinese Journal of Immunology,2010,26(4). |
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| Authors: | L Zhe WANG Wei FAN Yi-Qiang LIU Zhen-Long KONG Qing-Li WEN Ming-Jie LONG Jun LI Chen XU Qing AN Yun-Qing |
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| Institution: | L(U) Zhe,WANG Wei,FAN Yi-Qiang,LIU Zhen-Long,KONG Qing-Li,WEN Ming-Jie,LONG Jun,LI Chen,XU Qing,AN Yun-Qing |
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| Abstract: | Objective:To establish an experimental model for intracellular antibacteria and endotoxin neutralization in vitro to detect the antibacterial and endotoxin neutralization activity of the muBPI25 protein.Methods:RAW264.7 cells were transfected with pcDNA3.1(+)-muBPI36-259,and then were infected with intracellular bacterial of either G+/G to establish the experimental model of intracellular antibacteria.The RAW264.7 cells were co-transfected with the pSecTag2B-muBPI36-259 and dual-luciferase reporter gene pla... |
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| Keywords: | Bactericidal/permeability-increasing protein RAW264 7 cell line Intracelltdar bacterial parasite Endotoxin |
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