乳腺癌中Livin 和Survivin基因mRNA的表达及其临床意义

目的 探讨凋亡抑制基因Livin和Survivin在乳腺癌组织中的mRNA水平表达情况、两者间的相关性及其临床意义。方法 采用实时定量多聚酶链式反应(qRT-PCR)检测乳腺癌组织和癌旁正常组织中Livin和Survivin基因的mRNA表达情况。结果 61例乳腺癌中Survivin和Livin基因mRNA水平的阳性表达率分别是62.3%(38例)和54.1%(33例),Survivin的表达与临床分期、淋巴结转移和HER-2表达有关(P＜0.05),Livin的表达仅与淋巴结转移有关(P＜0.05)。在38例Survivin阳性表达病例中有21例Livin呈阳性表达,而两者之间不存在相关性(P＞0.05)。Survivin基因和Livin基因阳性表达患者3年总生存率明显低于阴性表达患者(P＜0.001和P＜0.001)。结论 乳腺癌组织中Survivin和Livin基因在mRNA水平表达上调与乳腺癌的发生发展密切相关,且两者与淋巴结转移的相关表明它们的高表达与乳腺癌较差预后有关,并可能成为乳腺癌中新的治疗靶点。

Expression level and its clinical significance of Livin and Survivin mRNA in human breast cancer

Purpose To explore the expression,relation and clinical significance of the apoptotic inhibitors Survivin and Livin mRNA in breast cancer. Method The mRNA expression of Surviving and Livin were detected in breast cancer samples and matched para - cancerous normal tissues by real - time quantitative RT - PCR（ qRT - PCR）. Results The positive rates for Survivin and Livin mRNA expressions were 62.3% （ 38 ） and 54.1% （33） in 61 breast cancer tissues, respectively. The significant correlation of Survivin was found with clinical stage,lymph node metastasis and HER - 2 expression （ P 〈 0.05 ）. And there was a relationship between the expression of Livin and lymph node metastasis. There was no correlation between mRNA expression of Survivin and Livin. It was shown that patients with high Survivin and Livin mRNA expression had significantly poorer over-all survival compared with patients with low expression of Livin and Survivin（ P 〈0.001 and P 〈0.001 ,respec-tively）. Conehtsions Our study suggests that up - regulated mRNA expression of Survivin and Livin genes may closely correlated with the carcinogenesis and development of breast cancer. The relationship between Survivin or Livin and lymph node metastasis indicates that higher Survivin and Livin expressions may imply patient＇s poor prognosis, and Survivin and Livin may serve as a new target for the therapy of breast cancer.