Inactivation of Proteolytic Enzymes from Porphyromonas gingivalis Using Light-activated Agents |
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Authors: | S Packer M Bhatti T Burns M Wilson |
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Institution: | (1) Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University of London, UK, GB;(2) Maxillofacial Surgery Research Unit, Eastman Dental Institute for Oral Health Care Sciences, University of London, UK, GB |
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Abstract: | . Previous studies have shown one of the causative agents of periodontitis, Porphyromonas gingivalis, can be killed by red light in the presence of the light-activated antimicrobial agent toluidine blue O (TBO). The purpose
of this study was to determine the effects of irradiating the organism with red light in the presence of TBO on its proteolytic
enzyme activity.
Suspensions of P. gingivalis were exposed to light with a wavelength of 633 nm in the presence of various concentrations of TBO. Samples were taken at
various times and their proteolytic activity determined by assay of azocasein hydrolysis. On exposure to 126 J of red light
in the presence of 12.5 μg/ml of TBO the proteolytic enzyme activity was reduced by 100%.
The results of this study have shown that the main virulence factor of P. gingivalis, its proteolytic activity, can be inactivated by red light in the presence of TBO. This, together with the known bactericidal
activity of the TBO/light combination, suggests that photodynamic therapy may prove important in reducing the effectiveness
of P. gingivalis as a periodontopathogen in vivo.
Paper received 24 June 1998; accepted after revision 20 June 1999. |
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Keywords: | : Bacteria Laser Lethal photosensitisation Periodontitis Photodynamic therapy Toluidine blue O |
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