| 苯并(a)芘对褐菖肝脏DNA损伤与抗氧活性的影响 |
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| 引用本文： | 王云,王顺昌,陆剑锋. 苯并(a)芘对褐菖肝脏DNA损伤与抗氧活性的影响[J]. 癌变．畸变．突变, 2009, 21(4) |
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| 作者姓名： | 王云 王顺昌 陆剑锋 |
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| 作者单位： | 1. 淮南师范学院生命科学系,安徽,淮南,232001
2. 合肥工业大学食品与生物工程学院,安微,合肥,230009 |
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| 基金项目： | 安徽省高校省级自然科学研究项目 |
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| 摘 要： | 背景与目的:研究苯并(a)芘BaP对褐菖鲉的毒性效应.材料与方法:将褐菖纳分别暴露于不同浓度(10、100、1 000 ng/L)的苯并(a)芘,0、7、25和50 d以及恢复期7、20 d取鱼肝脏,测定超氧化物歧化酶(SOD)、谷胱甘肽硫转移酶(GST)活性,还原型谷胱甘肽(GSH)含量和DNA单链断裂指标.实验同设溶剂对照组.结果:总SOD活性在Bap暴露7 d后被抑制,25 d后,10 ng/L和100ng/L Bap组SOD活性升高(P<0.05);50 d时,1 000 ng/L组SOD活性显著升高(P<0.05).10 ng/L BaP暴露7 d以及100 ng/L和1 000 ng/L BaP暴露50 d时,GSH含量显著增加(P<0.05).而GST活性在100 ng/L和1 000 ng/L BaP分别暴露25 d、50 d时显著增加,随着暴露时间的延长和暴露浓度的增加,各BaP浓度组DNA损伤呈加重趋势.结论:褐菖鲉肝脏SOD、GST酶活性与GSH含量结合使用以及DNA单链断裂损伤可以作为监测海洋环境中多环芳烃(PAHs)污染的潜在生物标志物.
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| 关 键 词： | 苯并(a)芘 褐菖刍由 抗氧化活性 DNA损伤 |
Impact of Benzo(a) pyrene on DNA Damage and Antioxidative Activities in Liver of Sebastiscus Marmoratus |
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| WANG Yun,WANG Shun-chang,LU Jian-feng. Impact of Benzo(a) pyrene on DNA Damage and Antioxidative Activities in Liver of Sebastiscus Marmoratus[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2009, 21(4) |
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| Authors: | WANG Yun WANG Shun-chang LU Jian-feng |
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| Affiliation: | WANG Yun1,WANG Shun-chang1,LU Jian-feng2(1.Department of Life Sciences,Huainan Normal University,Huainan 232001,Anhui,2.The School of Biotechnology , Food Engineering,Hefei University of Technology,Hefei 230009,China) |
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| Abstract: | BACKGROUND AND AIM: This study was designed to investigate antioxidative activities and DNA damage to determine toxicological effects of benzo(a)pyrene(BaP) on liver of Sebastiscus marmoratus.MATERIALS AND METHODS: Sebastiscus marmoratus were exposed through a water column to Bap(10,100,1 000 ng/L) and sampled at 0 d,7 d,25 d,50 d after exposure and 7 d,20 d after recovery.Activities of superoxide dismutase(SOD),glutathione S-transferase(GST),reduced glutathione(GSH) content and DNA single strand breaks wer... |
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| Keywords: | benzo(a)pyrene sebastiscus marmoratus antioxidative activities DNA damage |
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