呼畅清肺浓缩丸对肺炎支原体感染鼠免疫功能影响的研究

目的研究呼畅清肺浓缩丸提高肺炎支原体感染大鼠免疫功能的作用及机制。方法采用滴鼻法建立大鼠肺炎支原体感染模型,通过放射免疫法、速率散射比浊法和流式细胞仪技术研究呼畅清肺浓缩丸对模型大鼠体液免疫和细胞免疫功能的影响。结果与模型组比较,呼畅清肺浓缩丸能明显提高感染大鼠血清中IgG,IgM,IL2和IL6水平,降低TNFα和C₃补体含量,并使CD⁺₄/CD⁺₈比值增高。结论呼畅清肺浓缩丸能够明显改善支原体感染大鼠体液免疫和细胞免疫功能低下的状态,具有提高机体抗病原体感染的免疫作用。

Study of Huchang Qingfei pellets on immune function in rats infected with mycoplasma pneumoniae

OBJECTIVE: To study the effects and mechanism of Huchang Qingfei pellets on immune function in rats infected with mycoplasma pneumoniae. METHOD: A rat model of mycoplasmal pneumonia (MP) was developed in repeated intranasal infectious routes and then the humoral and cellular immunocompetences were detected by radioimmunoassay, immune-turbidimetry and flow cytometry. RESULT: The levels of serum IgG,IgM and IL-2, IL-6 were enhinced obviously, the complement C3 and TNF-alpha were decreased and the ratio of CD4+ /CD8+ was improved significantly in the Huchang groups as compared with MP model group. CONCLUSION: Huchang Qingfei pellets can reinforce immune function via preventing both cellular and humoral immunity from depression in the rats with MP.