| 外周血Bcl-2／IgH融合基因检测的可行性探讨 |
| |
| 引用本文： | 张学美,徐明,刘华,王玉明,李惠民,李云涛.外周血Bcl-2／IgH融合基因检测的可行性探讨[J].云南医药,2009(5):512-515. |
| |
| 作者姓名： | 张学美 徐明 刘华 王玉明 李惠民 李云涛 |
| |
| 作者单位： | 昆明医学院第一附属医院血液科及检验科,云南昆明650032 |
| |
| 摘 要： | 目的探讨外周血作为Bcl-2／IgH融合基因检测的标本来源的可行性。方法以SYBR Green I荧光染料实时定量PCR方法检测20例患者外周血和5例骨髓Bcl-2／IgH融合基因的阳性率及表达水平。结果骨髓和外周血Bcl-2／IgH融合基因的阳性检出率分别是80％与65％，两者阳性率经统计学分析差异无显著性（P=0．64）；外周血和骨髓融合基因相对拷贝数x^-±sd分别为2．73±2．54和4．23±4．06，经统计学分析差异无显著性（P=0．107）。结论以外周血作为Bcl-2／IgH融合基因观测的标本来源有一定的可行性，值得进一步研究。
|
| 关 键 词： | 淋巴瘤 外周血 Bcl-2/IgH融合基因 RQ—PCR |
Feasibility test on detection of Bel-2/IgH fusion gene using peripheral blood. |
| |
| Institution: | ZHANG Xue-mei, XU Ming, LIU Hua, et al. (Departments of Hematology and Clinical Laboratory, The First Affiliated Hospital of Kunraing Medical College, Kunming 650032, China) |
| |
| Abstract: | Objective To study the feasibility of peripheral blood （PB） as a source for detection of bcl-2/IgH fusion gene. Methods The Bcl-2/IgH positive rate and expression levels in 20 bone marrow （BM） and 5 peripheral blood （PB） were detected by using SYBR Green I real-time polymerase chain reaction. Results The positive rate of Bcl-2/IgH in BM and PB were 80% and 65% respectively. The relative copy-numbers of the Bcl-2/IgH fusion gene in BM and PB in Bcl-2/IgH-positive patients were 4.23±4.06 and 2.73± 2.54 respectively. There was no statistically significant difference in the positive rate （P=0.64） and relative copy-numbers （P=0.107） of Bcl-2/IgH between BM and PB. Conclusions The peripheral blood can be regarded as the source for detection of bcl-2/IgH fusion gene. |
| |
| Keywords: | Lymphoma Peripheral blood Bel-2/IgH fusion gene RQ-PCR |
| 本文献已被 维普 等数据库收录！ |
