Further characterization of Bacillus subtilis antibiotic biosensors and their use for antibacterial mode-of-action studies |
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Authors: | Mariner Katherine R Ooi Nicola Roebuck Deborah O'Neill Alex J Chopra Ian |
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Affiliation: | Antimicrobial Research Centre and Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom. |
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Abstract: | We further examined the usefulness of previously reported Bacillus subtilis biosensors for antibacterial mode-of-action studies. The biosensors could not detect the tRNA synthetase inhibitors mupirocin, indolmycin, and borrelidin, some inhibitors of peptidoglycan synthesis, and most membrane-damaging agents. However, the biosensors confirmed the modes of action of several RNA polymerase inhibitors and DNA intercalators and provided new insights into the possible modes of action of ciprofloxacin, anhydrotetracycline, corralopyronin, 8-hydroxyquinoline, and juglone. |
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