| Abstract: | An antibody against neurones, which has previously been demonstrated by immunofluorescence, is present in the sera of patients with sensory carcinomatous neuropathy. This antibody was demonstrated by a new technique, namely the use of purified peroxidase-labelled sheep anti-human γ-globulin. In cryostat sections of brain treated with a first layer of serum antibody followed by a second layer of peroxidase labelled anti-human γ-globulin, heavy coarse brown deposits in nerve cells only from different parts of the central nervous system indicated a positive reaction. The special technical precautions necessary to obtain specific staining of neurones are outlined. |
