| Hepatoprotective evaluation of Anogeissus latifolia： In vitro and in vivo studies |
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| 作者单位： | [1]Department of Pharmacology and Biotechnology, Nizam Institute of Pharmacy, Deshmukhi, Pocharnpally （Mandal）, Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India [2]JSS College of Pharmacy, Ooty, Tamilnadu, India and Manipal College of Pharmaceutical Sciences, Manipal 576104, Kamataka, India [3]Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad 500058, Andhra Pradesh, India |
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| 基金项目： | ACKNOWLEDGMENTS We are very grateful to Mr. Mohammed Jafer, Chairman, Nizam group of Institutions, Mr. Mohammed Fathe Azmath, Secretary, Nizam group of Institutions, and Mr P Chakrabarty, Director, Nizam group of Institutions, for patronage, encouragement, guidance, and financial support extended to us during the course of the study： |
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| 摘 要： | AIM： To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride （CCl4）. METHODS： In the in vitro study, a primary hepatocyte monolayer culture was treated with CCh and extract of Anogeissus latifolia. Hepatoprotective activity was demonstrated in the CCh damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract ofAnogeissus latifolia was analyzed in liver injured CCh-treated rats. Biochemical parameters including serum transaminases aspartate aminotransferase （AST） and alanine aminotransferase （ALT）] and alkaline phosphatase （ALP） in serum wereanalyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS： In vitro： primary hepatocyte monolayer cultures were treated with CCh and extract of Anogeissus latifolia. A protective activity could be demonstrated in the CCh damaged primary monolayer cultUre. In vivo： Hydroalcoholic extract of Anogeissus latifolia （300 mg/kg） was found to have protective activity in rats with CCh-induced liver damage as judged from serum marker enzyme activity. CONCLUSION： The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifol/a is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified.
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| 关 键 词： | 体外培养 肝细胞 体内 血清碱性磷酸酶 评价 丙氨酸转氨酶 组织病理学检查 单层培养 |
| 收稿时间： | 2009 Jun 13 |
Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies |
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| Authors: | Hulikere Ananth Pradeep Saleemullah Khan Karamkonda Ravikumar Mohammed Fazil Ahmed Meesala Srinivasa Rao Mandava Kiranmai Dachani Sudhershan Reddy Shaik Rasheed Ahamed Mohammed Ibrahim |
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| Institution: | Hulikere Ananth Pradeep；Saleemullah Khan；Karamkonda Ravikumar；Mohammed Fazil Ahmed；Meesala Srinivasa Rao；Mandava Kiranmai；Dachani Sudhershan Reddy；Shaik Rasheed Ahamed；Mohammed Ibrahim |
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| Abstract: | AIM: To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl_4). METHODS: In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl_4 and extract of Anogeissus latifolia. Hepatoprotective activity was demonstrated in the CCl_4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl_4-treated rats. Biochemical parameters including serum transaminases aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections. RESULTS: In vitro: primary hepatocyte monolayer cultures were treated with CCl_4 and extract of Anogeissus latifolia. A protective activity could be demonstrated in the CCl_4 damaged primary monolayer culture. In vivo: Hydroalcoholic extract of Anogeissus latifolia (300 mg/kg) was found to have protective activity in rats with CCl_4-induced liver damage as judged from serum marker enzyme activity. CONCLUSION: The above findings lead to the conclusion that the hydroalcoholic extract of Anogeissus latifolia is hepatoprotective. Hence, we suggest that the inclusion of this plant in the management of liver disorders is justified. |
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| Keywords: | Anogeissus latifolial Hepatoprotective Carbon tetrachloride |
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