Quantification of Iron-Labeled Cells with Positive Contrast in Mouse Brains |
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Authors: | Jean-Christophe Brisset Monica Sigovan Fabien Chauveau Adrien Riou Emilie Devillard Virginie Desestret Monique Touret Serge Nataf J Honnorat Emmanuelle Canet-Soulas Norbert Nighoghossian Yves Berthezene Marlene Wiart |
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Institution: | 1. Universit?? de Lyon, Lyon 1, Lyon, 69003, France 2. CNRS, UMR 5220; Inserm, U 630, Insa de Lyon; Creatis-LRMN, Bron, 69677, France 3. Inserm U842, NeuroOncologie and NeuroInflammation, Lyon, 69372, France 4. Laboratoire Creatis-LRMN CNRS-UMR 5220-U 630 Inserm, Service de radiologie (B13), Hopital Neuro-Cardiologique, 28, avenue du Doyen LEPINE, 69677, Bron Cedex, France
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Abstract: | Purpose To quantify small amounts of iron-labeled cells in mouse brains with magnetic resonance imaging (MRI). Procedures Iron-labeled cells (from 500 to 7,500) were stereotaxically transplanted into the brain of living mice that were subsequently imaged with MRI at 4.7 T. We compared four quantitative methods: (1) T2 relaxometry, (2) T2* relaxometry, (3) the volume of the cloverleaf hypointense artifact generated on T2*-weighted images, and (4) the volume of the cloverleaf hyperintense artifact generated on positive contrast images. Results The methods based on relaxometry, whether T2 or T2*, did not correlate with the number of injected cells. By contrast, those based on measurement of cloverleaf artifact volume, whether using negative or positive enhancement, showed a significant linear relationship for the given range of cells (R 0.92?C0.95], p?<?0.05). Conclusions T2* artifact volume imaging (negative or positive) appears promising for the quantification of magnetically labeled cells following focal injection in the brain. |
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